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Anti-Human SLC12A2 Polyclonal Antibody (HX014014)

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Overview
Catalog No.HX014014
Description
Anti-Human SLC12A2 Polyclonal Antibody (HX014014) is a rabbit polyclonal antibody detecting SLC12A2 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human SLC12A2 (Asn1002-Ser1212).
Target Solute carrier family 12 member 2, Basolateral Na-K-Cl symporter, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2, SLC12A2, NKCC1
Purification Purified by antigen affinity column.
Accession P55011
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Solute carrier family 12 member 2 (SLC12A2/NKCC1) is a ~131 kDa protein. Cation-chloride cotransporter which mediates the electroneutral transport of chloride, potassium and/or sodium ions across the membrane. Plays a vital role in the regulation of ionic balance and cell volume.

1. Vitari, AC. et al. (2006) The Biochemical journal 397, 223-31. PMID: 16669787
2. Yang, X. et al. (2020) Nature communications 11, 1016. PMID: 32081947
3. Mutai, H. et al. (2020) PLoS genetics 16, e1008643. PMID: 32294086
4. Zhang, S. et al. (2021) Communications biology 4, 226. PMID: 33597714
5. Zhao, Y. et al. (2022) Nature communications 13, 2747. PMID: 35585053
6. Neumann, C. et al. (2022) The EMBO journal 41, e110169. PMID: 36239040
7. Moseng, MA. et al. (2022) Science advances 8, eabq0952. PMID: 36306358
8. Payne, JA. et al. (1995) The Journal of biological chemistry 270, 17977-85. PMID: 7629105
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human SLC12A2 Polyclonal Antibody

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