

| Каталожный номер | HF639012 |
|---|---|
| Описание |
Recombinant Human SLC25A12 Protein, N-His (HF639012) expressed in E. coli, spanning Gly11-Gln311. Purity: >90% by SDS-PAGE.
Highlights
|
| Система экспрессии | E. coli |
| Номер доступа | O75746 |
| Длина белка | Gly11-Gln311 |
| Применения | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Виды | Homo sapiens (Human) |
| Характер | Recombinant |
| Уровень эндотоксинов | Please contact with the lab for this information. |
| Чистота | >90% as determined by SDS-PAGE. |
| Прогнозируемая молекулярная масса | 36.56 kDa |
| Форма | Lyophilized |
| Буфер хранения | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии. |
| Реконституция | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| Доставка | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Устойчивость и хранение | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Альтернативные названия | Electrogenic aspartate/glutamate antiporter SLC25A12, mitochondrial, Araceli hiperlarga, Aralar, Aralar1, Mitochondrial aspartate glutamate carrier 1, Solute carrier family 25 member 12, SLC25A12, AGC1, ARALAR1 |
| Фон | Electrogenic aspartate/glutamate antiporter SLC25A12, mitochondrial is a ~74 kDa protein. Mitochondrial electrogenic aspartate/glutamate antiporter that favors efflux of aspartate and entry of glutamate and proton within the mitochondria as part of the malate-aspartate shuttle. Also mediates the uptake of L-cysteinesulfinate (3-sulfino-L-alanine) by mitochondria in exchange of L-glutamate and proton. Can also exchange L-cysteinesulfinate with aspartate in their anionic form without any proton translocation. Lacks transport activity towards L-glutamine or gamma-aminobutyric acid (GABA). 1. Palmieri, L. et al. (2001) The EMBO journal 20, 5060-9. PMID: 11566871 2. Wibom, R. et al. (2009) The New England journal of medicine 361, 489-95. PMID: 19641205 3. Falk, MJ. et al. (2014) JIMD reports 14, 77-85. PMID: 24515575 4. Porcelli, V. et al. (2024) Biochimica et biophysica acta. Bioenergetics 1865, 149487. PMID: 38945283 |
| Примечание | For research use only. |

SDS-PAGE for Recombinant Human SLC25A12 Protein, N-His
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