Please ensure Javascript is enabled for purposes of website accessibility
Главная > Продукты > Рекомбинантные Белки > Прочие белки
Human SLC7A5 Recombinant Protein (N-GST & C-His)
Human SLC7A5 Recombinant Protein (N-GST & C-His)
Цена(USD):
Спецификация:
  • 50ug
  • 100ug
  • 1mg
Количество:
Свяжитесь с нами
  • Обзор

  • Изображения

  • Ссылки

  • Даташит

  • SDS

Обзор
Каталожный номерHC031012
Описание
Recombinant Human SLC7A5 Protein, N-GST & C-His (HC031012) expressed in E. coli. Purity: >90% by SDS-PAGE.
Highlights
  • ●His-Tagged — N-terminal 6×His tag for IMAC purification.
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессииE. coli
Номер доступаQ01650
Длина белкаVal148-Glu169&Gly215-Asn242&Thr298-Ser324&Lys479-Thr507
ПримененияELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
ВидыHomo sapiens (Human)
Характер Recombinant
Уровень эндотоксинов Please contact with the lab for this information.
Чистота >90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса 40.21 kDa
Форма Lyophilized
Буфер хранения Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Реконституция Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.

RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названияLarge neutral amino acids transporter small subunit 1; 4F2 light chain; 4F2 LC; 4F2LC; CD98 light chain; Integral membrane protein E16; E16; L-type amino acid transporter 1; hLAT1; Solute carrier family 7 member 5; y+ system cationic amino acid transporter; SLC7A5; CD98LC; LAT1; MPE16
Фон

Large neutral amino acids transporter small subunit 1 is a ~55 kDa protein. The heterodimer with SLC3A2 functions as a sodium-independent, high-affinity transporter that mediates uptake of large neutral amino acids such as phenylalanine, tyrosine, leucine, histidine, methionine, tryptophan, valine, isoleucine and alanine. The heterodimer with SLC3A2 mediates the uptake of L-DOPA. Functions as an amino acid exchanger. May play a role in the transport of L-DOPA across the blood-brain barrier. May act as the major transporter of tyrosine in fibroblasts (Probable).

1. Prasad, PD. et al. (1999) Biochemical and biophysical research communications 255, 283-8. PMID: 10049700
2. Rossier, G. et al. (1999) The Journal of biological chemistry 274, 34948-54. PMID: 10574970
3. Yanagida, O. et al. (2001) Biochimica et biophysica acta 1514, 291-302. PMID: 11557028
4. Friesema, EC. et al. (2001) Endocrinology 142, 4339-48. PMID: 11564694
5. Simmons-Willis, TA. et al. (2002) The Biochemical journal 367, 239-46. PMID: 12117417
6. Kim, DK. et al. (2002) Biochimica et biophysica acta 1565, 112-21. PMID: 12225859
7. Li, S. et al. (2005) The Journal of biological chemistry 280, 20102-10. PMID: 15769744
8. Vumma, R. et al. (2008) Neuroscience letters 434, 56-60. PMID: 18262359
9. Milkereit, R. et al. (2015) Nature communications 6, 7250. PMID: 25998567
10. Yan, R. et al. (2019) Nature 568, 127-130. PMID: 30867591
Примечание For research use only
Изображения
  • Human SLC7A5 Recombinant Protein (N-GST & C-His)

    SDS-PAGE

    SDS-PAGE for Recombinant Human SLC7A5 Protein, N-GST & C-His

Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Рекомендация

Свяжитесь с нами для индивидуальных предложений, оптовых запросов и других вопросов.

Почта: [email protected]

Список дистрибьюторов