Anti-Human SLC7A5 Polyclonal Antibody (HC031014)

Name: Anti-Human SLC7A5 Polyclonal Antibody
Brand: abinScience
SKU: HC031014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HC031014

Description

Anti-Human SLC7A5 Polyclonal Antibody (HC031014) is a rabbit polyclonal antibody detecting Large neutral amino acids transporter small subunit 1; 4F2 light chain; 4F2 LC; 4F2LC; CD98 light chain; Integral membrane protein E16; E16; L-type amino acid transporter 1; hLAT1; Solute carrier family 7 member 5; y+ system cationic amino acid transporter; SLC7A5; CD98LC; LAT1; MPE16 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant human SLC7A5 (Val148-Glu169&Gly215-Asn242&Thr298-Ser324&Lys479-Thr507).

Target

Large neutral amino acids transporter small subunit 1; 4F2 light chain; 4F2 LC; 4F2LC; CD98 light chain; Integral membrane protein E16; E16; L-type amino acid transporter 1; hLAT1; Solute carrier family 7 member 5; y+ system cationic amino acid transporter; SLC7A5; CD98LC; LAT1; MPE16

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q01650

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Large neutral amino acids transporter small subunit 1 (SLC7A5) is a ~55 kDa protein. The heterodimer with SLC3A2 functions as a sodium-independent, high-affinity transporter that mediates uptake of large neutral amino acids such as phenylalanine, tyrosine, leucine, histidine, methionine, tryptophan, valine, isoleucine and alanine. The heterodimer with SLC3A2 mediates the uptake of L-DOPA. Functions as an amino acid exchanger. May play a role in the transport of L-DOPA across the blood-brain barrier. May act as the major transporter of tyrosine in fibroblasts (Probable).

1. Prasad, PD. et al. (1999) Biochemical and biophysical research communications 255, 283-8. PMID: 10049700

2. Rossier, G. et al. (1999) The Journal of biological chemistry 274, 34948-54. PMID: 10574970

3. Yanagida, O. et al. (2001) Biochimica et biophysica acta 1514, 291-302. PMID: 11557028

4. Friesema, EC. et al. (2001) Endocrinology 142, 4339-48. PMID: 11564694

5. Simmons-Willis, TA. et al. (2002) The Biochemical journal 367, 239-46. PMID: 12117417

6. Kim, DK. et al. (2002) Biochimica et biophysica acta 1565, 112-21. PMID: 12225859

7. Li, S. et al. (2005) The Journal of biological chemistry 280, 20102-10. PMID: 15769744

8. Vumma, R. et al. (2008) Neuroscience letters 434, 56-60. PMID: 18262359

9. Milkereit, R. et al. (2015) Nature communications 6, 7250. PMID: 25998567

10. Yan, R. et al. (2019) Nature 568, 127-130. PMID: 30867591

Note

For research use only

Images

Western blot

Lane 1: A549 cell lysate
Lane 2: Hela cell lysate
Lane 3: MCF-7 cell lysate
Lane 4: HepG2 cell lysate

References

Datasheet

Anti-Human SLC7A5 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human SLC7A5 Polyclonal Antibody (HC031014)

Overview

Images

References

Datasheet

SDS

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