

| Número de catálogo | HF704012 |
|---|---|
| Descripción |
Recombinant Human SLU7 Protein, N-His (HF704012) expressed in E. coli, spanning Met28-Tyr173. Purity: >90% by SDS-PAGE.
Highlights
|
| Sistema de expresión | E. coli |
| Número de acceso | O95391 |
| Longitud de proteína | Met28-Tyr173 |
| Aplicaciones | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Especies | Homo sapiens (Human) |
| Naturaleza | Recombinant |
| Nivel de endotoxinas | Please contact with the lab for this information. |
| Pureza | >90% as determined by SDS-PAGE. |
| Peso molecular predicho | 19.37 kDa |
| Forma | Lyophilized |
| Buffer de almacenamiento | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote. |
| Reconstitución | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| Envío | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Estabilidad y almacenamiento | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Nombres alternativos | Pre-mRNA-splicing factor SLU7, hSlu7, SLU7 |
| Fondo | Pre-mRNA-splicing factor SLU7 is a ~68 kDa protein. Required for pre-mRNA splicing as component of the spliceosome. Participates in the second catalytic step of pre-mRNA splicing, when the free hydroxyl group of exon I attacks the 3'-splice site to generate spliced mRNA and the excised lariat intron. Required for holding exon 1 properly in the spliceosome and for correct AG identification when more than one possible AG exists in 3'-splicing site region. May be involved in the activation of proximal AG. Probably also involved in alternative splicing regulation. 1. Chua, K. et al. (1999) Genes & development 13, 841-50. PMID: 10197984 2. Zhang, X. et al. (2017) Cell 169, 918-929.e14. PMID: 28502770 3. Fica, SM. et al. (2019) Science (New York, N.Y.) 363, 710-714. PMID: 30705154 |
| Nota | For research use only. |

SDS-PAGE for Recombinant Human SLU7 Protein, N-His
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