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Anti-Human SLU7 Polyclonal Antibody (HF704014)

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Overview
Catalog No.HF704014
Description
Anti-Human SLU7 Polyclonal Antibody (HF704014) is a rabbit polyclonal antibody detecting SLU7 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human SLU7 (Met28-Tyr173).
Target Pre-mRNA-splicing factor SLU7, hSlu7, SLU7
Purification Purified by antigen affinity column.
Accession O95391
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Pre-mRNA-splicing factor SLU7 is a ~68 kDa protein. Required for pre-mRNA splicing as component of the spliceosome. Participates in the second catalytic step of pre-mRNA splicing, when the free hydroxyl group of exon I attacks the 3'-splice site to generate spliced mRNA and the excised lariat intron. Required for holding exon 1 properly in the spliceosome and for correct AG identification when more than one possible AG exists in 3'-splicing site region. May be involved in the activation of proximal AG. Probably also involved in alternative splicing regulation.

1. Chua, K. et al. (1999) Genes & development 13, 841-50. PMID: 10197984
2. Zhang, X. et al. (2017) Cell 169, 918-929.e14. PMID: 28502770
3. Fica, SM. et al. (2019) Science (New York, N.Y.) 363, 710-714. PMID: 30705154
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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Anti-Human SLU7 Polyclonal Antibody

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