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| カタログ番号 | HV674012 |
|---|---|
| 説明 |
Recombinant Human SPHK1 Protein, N-His (HV674012) expressed in E. coli, spanning Ser148-Leu384. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q9NYA1 |
| タンパク質長 | Ser148-Leu384 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 28.63 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | SPHK, SK 1, SPK, Acetyltransferase SPHK1, Sphingosine kinase 1, SPHK1, SPK 1, SK1 |
| 背景 | Sphingosine kinase 1 (SPHK1) is a ~42 kDa protein. Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. In contrast to proapoptotic SPHK2, has a negative effect on intracellular ceramide levels, enhances cell growth and inhibits apoptosis. Involved in the regulation of inflammatory response and neuroinflammation. Via the product sphingosine 1-phosphate, stimulates TRAF2 E3 ubiquitin ligase activity, and promotes activation of NF-kappa-B in response to TNF signaling leading to IL17 secretion. 1. Gijsbers, S. et al. (2002) Biochimica et biophysica acta 1580, 1-8. PMID: 11923095 2. Alvarez, SE. et al. (2010) Nature 465, 1084-8. PMID: 20577214 3. Wang, Z. et al. (2013) Structure (London, England : 1993) 21, 798-809. PMID: 23602659 4. Shen, H. et al. (2014) Nature cell biology 16, 652-62. PMID: 24929359 5. Lee, JY. et al. (2018) Nature communications 9, 1479. PMID: 29662056 6. Maceyka, M. et al. (2005) The Journal of biological chemistry 280, 37118-29. PMID: 16118219 7. Adada, MM. et al. (2013) The Journal of biological chemistry 288, 27667-27679. PMID: 23935096 8. Lima, S. et al. (2017) The Journal of biological chemistry 292, 3074-3088. PMID: 28049734 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human SPHK1 Protein, N-His
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