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Anti-SPHK1 Polyclonal Antibody (HV674014)

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Overview
Catalog No.HV674014
Description
Anti-SPHK1 Polyclonal Antibody (HV674014) is a rabbit polyclonal antibody detecting SPHK1 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human SPHK1 (Ser148-Leu384).
Target SPHK, SK 1, SPK, Acetyltransferase SPHK1, Sphingosine kinase 1, SPHK1, SPK 1, SK1
Purification Purified by antigen affinity column.
Accession Q9NYA1
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Sphingosine kinase 1 (SPHK1) is a ~42 kDa protein. Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. In contrast to proapoptotic SPHK2, has a negative effect on intracellular ceramide levels, enhances cell growth and inhibits apoptosis. Involved in the regulation of inflammatory response and neuroinflammation. Via the product sphingosine 1-phosphate, stimulates TRAF2 E3 ubiquitin ligase activity, and promotes activation of NF-kappa-B in response to TNF signaling leading to IL17 secretion.

1. Gijsbers, S. et al. (2002) Biochimica et biophysica acta 1580, 1-8. PMID: 11923095
2. Alvarez, SE. et al. (2010) Nature 465, 1084-8. PMID: 20577214
3. Wang, Z. et al. (2013) Structure (London, England : 1993) 21, 798-809. PMID: 23602659
4. Shen, H. et al. (2014) Nature cell biology 16, 652-62. PMID: 24929359
5. Lee, JY. et al. (2018) Nature communications 9, 1479. PMID: 29662056
6. Maceyka, M. et al. (2005) The Journal of biological chemistry 280, 37118-29. PMID: 16118219
7. Adada, MM. et al. (2013) The Journal of biological chemistry 288, 27667-27679. PMID: 23935096
8. Lima, S. et al. (2017) The Journal of biological chemistry 292, 3074-3088. PMID: 28049734
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-SPHK1 Polyclonal Antibody

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