Human SPOUT1 Recombinant Protein (N-His) (HD297012)

Name: Human SPOUT1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HD297012
Price: 211 USD
Availability: InStock

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Número de catálogo	HD297012
Descripción	Recombinant Human SPOUT1 Protein, N-His (HD297012) expressed in E. coli, spanning Thr74-Thr376. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	Q5T280
Longitud de proteína	Thr74-Thr376
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Homo sapiens (Human)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	35.35 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	28S rRNA (uridine-N(3))-methyltransferase, C9orf114, CENP-32, Centromere protein 32, EC:2.1.1.-, Kinetochore-associated protein, Methyltransferase C9orf114, Ribosomal RNA methyltransferase SPOUT1, SPOUT domain-containing methyltransferase 1, SPOUT1
Fondo	28S rRNA (uridine-N(3))-methyltransferase is a ~42 kDa protein. S-adenosyl-L-methionine-dependent RNA methyltransferase. Specifically methylates the N3 position of a uridine in 28S rRNA. Required for association of the centrosomes with the poles of the bipolar mitotic spindle during metaphase, ensuring proper spindle organization. Also involved in chromosome alignment. May promote centrosome maturation probably by recruiting A-kinase anchor protein AKAP9 to centrosomes in early mitosis. 1. Chen, J. et al. (2024) Science advances 10, eadr1743. PMID: 39671501 2. Dharmadhikari, AV. et al. (2025) Nature communications 16, 1703. PMID: 39962046 3. Ohta, S. et al. (2010) Cell 142, 810-21. PMID: 20813266 4. Ohta, S. et al. (2015) Molecular biology of the cell 26, 1225-37. PMID: 25657325 5. Treiber, T. et al. (2017) Molecular cell 66, 270-284.e13. PMID: 28431233
Nota	For research use only

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Datasheet

Human SPOUT1 Recombinant Protein (N-His).pdf

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