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Anti-Human SPOUT1 Polyclonal Antibody (HD297014)

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Overview
Catalog No.HD297014
Description
Anti-Human SPOUT1 Polyclonal Antibody (HD297014) is a rabbit polyclonal antibody detecting SPOUT1. Suitable for Mouse and Human.
Highlights
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human SPOUT1 (Thr74-Thr376).
Target 28S rRNA (uridine-N(3))-methyltransferase, C9orf114, CENP-32, Centromere protein 32, EC:2.1.1.-, Kinetochore-associated protein, Methyltransferase C9orf114, Ribosomal RNA methyltransferase SPOUT1, SPOUT domain-containing methyltransferase 1, SPOUT1
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q5T280
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

28S rRNA (uridine-N(3))-methyltransferase (SPOUT1) is a ~42 kDa protein. S-adenosyl-L-methionine-dependent RNA methyltransferase. Specifically methylates the N3 position of a uridine in 28S rRNA. Required for association of the centrosomes with the poles of the bipolar mitotic spindle during metaphase, ensuring proper spindle organization. Also involved in chromosome alignment. May promote centrosome maturation probably by recruiting A-kinase anchor protein AKAP9 to centrosomes in early mitosis.

1. Chen, J. et al. (2024) Science advances 10, eadr1743. PMID: 39671501
2. Dharmadhikari, AV. et al. (2025) Nature communications 16, 1703. PMID: 39962046
3. Ohta, S. et al. (2010) Cell 142, 810-21. PMID: 20813266
4. Ohta, S. et al. (2015) Molecular biology of the cell 26, 1225-37. PMID: 25657325
5. Treiber, T. et al. (2017) Molecular cell 66, 270-284.e13. PMID: 28431233
Note For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-Human SPOUT1 Polyclonal Antibody

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