Human SPOUT1 Recombinant Protein (N-His) (HD297012)

Name: Human SPOUT1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HD297012
Price: 211 USD
Availability: InStock

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Numéro de catalogue	HD297012
Description	Recombinant Human SPOUT1 Protein, N-His (HD297012) expressed in E. coli, spanning Thr74-Thr376. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression	E. coli
Numéro d'accès	Q5T280
Longueur de protéine	Thr74-Thr376
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces	Homo sapiens (Human)
Nature	Recombinant
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>90% as determined by SDS-PAGE.
Masse moléculaire prédite	35.35 kDa
Forme	Lyophilized
Tampon de stockage	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Expédition	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	28S rRNA (uridine-N(3))-methyltransferase, C9orf114, CENP-32, Centromere protein 32, EC:2.1.1.-, Kinetochore-associated protein, Methyltransferase C9orf114, Ribosomal RNA methyltransferase SPOUT1, SPOUT domain-containing methyltransferase 1, SPOUT1
Contexte	28S rRNA (uridine-N(3))-methyltransferase is a ~42 kDa protein. S-adenosyl-L-methionine-dependent RNA methyltransferase. Specifically methylates the N3 position of a uridine in 28S rRNA. Required for association of the centrosomes with the poles of the bipolar mitotic spindle during metaphase, ensuring proper spindle organization. Also involved in chromosome alignment. May promote centrosome maturation probably by recruiting A-kinase anchor protein AKAP9 to centrosomes in early mitosis. 1. Chen, J. et al. (2024) Science advances 10, eadr1743. PMID: 39671501 2. Dharmadhikari, AV. et al. (2025) Nature communications 16, 1703. PMID: 39962046 3. Ohta, S. et al. (2010) Cell 142, 810-21. PMID: 20813266 4. Ohta, S. et al. (2015) Molecular biology of the cell 26, 1225-37. PMID: 25657325 5. Treiber, T. et al. (2017) Molecular cell 66, 270-284.e13. PMID: 28431233
Note	For research use only

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Human SPOUT1 Recombinant Protein (N-His).pdf

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