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| カタログ番号 | HY387012 |
|---|---|
| 説明 |
Recombinant Human SRC Protein, N-His (HY387012) expressed in E. coli, spanning Tyr187-Leu536. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | P12931 |
| タンパク質長 | Tyr187-Leu536 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 42.21 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | SRC1, p60-Src, Proto-oncogene tyrosine-protein kinase Src, Proto-oncogene c-Src, SRC, pp60c-src |
| 背景 | Proto-oncogene tyrosine-protein kinase Src is a ~59 kDa protein. Non-receptor protein tyrosine kinase which is activated following engagement of many different classes of cellular receptors including immune response receptors, integrins and other adhesion receptors, receptor protein tyrosine kinases, G protein-coupled receptors as well as cytokine receptors. Participates in signaling pathways that control a diverse spectrum of biological activities including gene transcription, immune response, cell adhesion, cell cycle progression, apoptosis, migration, and transformation. Due to functional redundancy between members of the SRC kinase family, identification of the specific role of each SRC kinase is very difficult. SRC appears to be one of the primary kinases activated following engagement of receptors and plays a role in the activation of other protein tyrosine kinase (PTK) families. Receptor clustering or dimerization leads to recruitment of SRC to the receptor complexes where it phosphorylates the tyrosine residues within the receptor cytoplasmic domains. 1. Paré, G. et al. (2021) Frontiers in immunology 12, 650808. PMID: 34234773 2. Wang, Y. et al. (2011) Molecular biology of the cell 22, 1529-38. PMID: 21411625 3. Neuber, S. et al. (2015) Cell and tissue research 359, 799-816. PMID: 25501895 4. Giglione, C. et al. (2001) European journal of biochemistry 268, 3275-83. PMID: 11389730 5. Jeulin, C. et al. (2008) American journal of physiology. Lung cellular and molecular physiology 295, L489-96. PMID: 18586953 6. David-Pfeuty, T. et al. (1995) Journal of virology 69, 1699-713. PMID: 7853507 7. Taniyama, Y. et al. (2003) Molecular and cellular biology 23, 8019-29. PMID: 14585963 8. Grano, M. et al. (1996) Proceedings of the National Academy of Sciences of the United States of America 93, 7644-8. PMID: 8755529 9. Li, Z. et al. (2024) Nature communications 15, 1300. PMID: 38346942 10. Miyazaki, T. et al. (2003) The Journal of cell biology 160, 709-18. PMID: 12615910 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human SRC Protein, N-His
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中国武漢市深敦寺路666号C棟、武漢、430206
