Human SUCLA2 Recombinant Protein (N-His) (HP689012)

Name: Human SUCLA2 Recombinant Protein (N-His)
Brand: abinScience
SKU: HP689012
Price: 211 USD
Availability: InStock

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Aperçu

Numéro de catalogue	HP689012
Description	Recombinant Human SUCLA2 Protein, N-His (HP689012) expressed in E. coli, spanning Ser54-Glu445. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression	E. coli
Numéro d'accès	Q9P2R7
Longueur de protéine	Ser54-Glu445
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces	Homo sapiens (Human)
Nature	Recombinant
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>90% as determined by SDS-PAGE.
Masse moléculaire prédite	44.76 kDa
Forme	Lyophilized
Tampon de stockage	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	ATP-specific succinyl-CoA synthetase subunit beta, SCS-betaA, Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial, A-SCS, Succinyl-CoA synthetase beta-A chain, SUCLA2
Contexte	Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial (SUCLA2) is a ~50 kDa protein. ATP-specific succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of ATP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit. Also able to act as an ATP-specific itaconyl- and malyl-CoA synthetase. 1. Elpeleg, O. et al. (2005) American journal of human genetics 76, 1081-6. PMID: 15877282 2. Marcero, JR. et al. (2021) Blood advances 5, 4831-4841. PMID: 34492704 3. Griffith, CM. et al. (2025) Nature chemical biology 21, 906-915. PMID: 40108300
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human SUCLA2 Protein, N-His

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Human SUCLA2 Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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