ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies
Homo sapiens (Human)
Naturaleza
Recombinant
Nivel de endotoxinas
Please contact with the lab for this information.
Pureza
>90% as determined by SDS-PAGE.
Peso molecular predicho
33.22 kDa
Forma
Lyophilized
Buffer de almacenamiento
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos
TAP1, APT1, Peptide transporter involved in antigen processing 1, Really interesting new gene 4 protein, Peptide transporter TAP1, Peptide transporter PSF1, RING4, Antigen peptide transporter 1, PSF-1, ABCB2, Y3, Peptide supply factor 1, PSF1, ATP-binding cassette sub-family B member 2
Fondo
Antigen peptide transporter 1 (TAP1) is a ~80 kDa protein. ABC transporter associated with antigen processing. In complex with TAP2 mediates unidirectional translocation of peptide antigens from cytosol to endoplasmic reticulum (ER) for loading onto MHC class I (MHCI) molecules. Uses the chemical energy of ATP to export peptides against the concentration gradient. During the transport cycle alternates between 'inward-facing' state with peptide binding site facing the cytosol to 'outward-facing' state with peptide binding site facing the ER lumen. Peptide antigen binding to ATP-loaded TAP1-TAP2 induces a switch to hydrolysis-competent 'outward-facing' conformation ready for peptide loading onto nascent MHCI molecules.
1. Grossmann, N. et al. (2014) Nature communications 5, 5419. PMID: 25377891
2. Fischbach, H. et al. (2015) Nature communications 6, 6199. PMID: 25656091
3. Gorbulev, S. et al. (2001) Proceedings of the National Academy of Sciences of the United States of America 98, 3732-7. PMID: 11274390
4. van Endert, PM. et al. (1995) The Journal of experimental medicine 182, 1883-95. PMID: 7500034
5. Uebel, S. et al. (1997) Proceedings of the National Academy of Sciences of the United States of America 94, 8976-81. PMID: 9256420
6. Kelly, A. et al. (1992) Nature 355, 641-4. PMID: 1538751
Nota
For research use only.
Imágenes
SDS-PAGE
SDS-PAGE for Recombinant Human TAP1 Protein, N-His
中文
English
한국어
日本語
Español
Français
Русский
