Anti-TAP1 Polyclonal Antibody (HX909014)

Name: Anti-TAP1 Polyclonal Antibody
Brand: abinScience
SKU: HX909014
Price: 168 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD):

Spec:

50ug
100ug

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HX909014

Description

Anti-TAP1 Polyclonal Antibody (HX909014) is a rabbit polyclonal antibody detecting TAP1 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human TAP1 (Tyr525-Glu808).

Target

TAP1, APT1, Peptide transporter involved in antigen processing 1, Really interesting new gene 4 protein, Peptide transporter TAP1, Peptide transporter PSF1, RING4, Antigen peptide transporter 1, PSF-1, ABCB2, Y3, Peptide supply factor 1, PSF1, ATP-binding cassette sub-family B member 2

Purification

Purified by antigen affinity column.

Accession

Q03518

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Antigen peptide transporter 1 (TAP1) is a ~80 kDa protein. ABC transporter associated with antigen processing. In complex with TAP2 mediates unidirectional translocation of peptide antigens from cytosol to endoplasmic reticulum (ER) for loading onto MHC class I (MHCI) molecules. Uses the chemical energy of ATP to export peptides against the concentration gradient. During the transport cycle alternates between 'inward-facing' state with peptide binding site facing the cytosol to 'outward-facing' state with peptide binding site facing the ER lumen. Peptide antigen binding to ATP-loaded TAP1-TAP2 induces a switch to hydrolysis-competent 'outward-facing' conformation ready for peptide loading onto nascent MHCI molecules.

1. Grossmann, N. et al. (2014) Nature communications 5, 5419. PMID: 25377891

2. Fischbach, H. et al. (2015) Nature communications 6, 6199. PMID: 25656091

3. Gorbulev, S. et al. (2001) Proceedings of the National Academy of Sciences of the United States of America 98, 3732-7. PMID: 11274390

4. van Endert, PM. et al. (1995) The Journal of experimental medicine 182, 1883-95. PMID: 7500034

5. Uebel, S. et al. (1997) Proceedings of the National Academy of Sciences of the United States of America 94, 8976-81. PMID: 9256420

6. Kelly, A. et al. (1992) Nature 355, 641-4. PMID: 1538751

Note

For research use only.

Images

References

Datasheet

Anti-TAP1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-TAP1 Polyclonal Antibody (HX909014)

Overview

Images

References

Datasheet

SDS

Quick access

Contact Us