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| カタログ番号 | HX990012 |
|---|---|
| 説明 |
Recombinant Human TFAM Protein, N-GST (HX990012) expressed in E. coli, spanning Ser43-Cys246. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q00059 |
| タンパク質長 | Ser43-Cys246 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 51.22 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | Transcription factor 6-like 2, Mitochondrial transcription factor 1, MtTF1, mtTFA, Transcription factor 6, TCF6, TCF6L2, Transcription factor A, mitochondrial, TCF-6, TFAM |
| 背景 | Transcription factor A, mitochondrial (TFAM) is a ~29 kDa protein. Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Component of the mitochondrial transcription initiation complex, composed at least of TFB2M, TFAM and POLRMT that is required for basal transcription of mitochondrial DNA. In this complex, TFAM recruits POLRMT to a specific promoter whereas TFB2M induces structural changes in POLRMT to enable promoter opening and trapping of the DNA non-template strand. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. 1. Celestini, V. et al. (2018) Cell death & disease 9, 231. PMID: 29445193 2. Hao, Z. et al. (2020) Molecular cell 78, 382-395.e8. PMID: 32183942 3. Hillen, HS. et al. (2017) Cell 171, 1072-1081.e10. PMID: 29149603 4. Litonin, D. et al. (2010) The Journal of biological chemistry 285, 18129-33. PMID: 20410300 5. Rubio-Cosials, A. et al. (2011) Nature structural & molecular biology 18, 1281-9. PMID: 22037172 6. Fisher, RP. et al. (1992) The Journal of biological chemistry 267, 3358-67. PMID: 1737790 7. Gangelhoff, TA. et al. (2009) Nucleic acids research 37, 3153-64. PMID: 19304746 8. Kasashima, K. et al. (2012) Experimental cell research 318, 2335-43. PMID: 22841477 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human TFAM Protein, N-GST
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中国武漢市深敦寺路666号C棟、武漢、430206
