Anti-Human TFAM Polyclonal Antibody (HX990014)

Name: Anti-Human TFAM Polyclonal Antibody
Brand: abinScience
SKU: HX990014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HX990014

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human TFAM (Ser43-Cys246).

Target

Transcription factor 6-like 2, Mitochondrial transcription factor 1, MtTF1, mtTFA, Transcription factor 6, TCF6, TCF6L2, Transcription factor A, mitochondrial, TCF-6, TFAM

Purification

Purified by antigen affinity column.

Accession

Q00059

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Transcription factor A, mitochondrial (TFAM) is a ~29 kDa protein. Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Component of the mitochondrial transcription initiation complex, composed at least of TFB2M, TFAM and POLRMT that is required for basal transcription of mitochondrial DNA. In this complex, TFAM recruits POLRMT to a specific promoter whereas TFB2M induces structural changes in POLRMT to enable promoter opening and trapping of the DNA non-template strand. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites.

1. Celestini, V. et al. (2018) Cell death & disease 9, 231. PMID: 29445193

2. Hao, Z. et al. (2020) Molecular cell 78, 382-395.e8. PMID: 32183942

3. Hillen, HS. et al. (2017) Cell 171, 1072-1081.e10. PMID: 29149603

4. Litonin, D. et al. (2010) The Journal of biological chemistry 285, 18129-33. PMID: 20410300

5. Rubio-Cosials, A. et al. (2011) Nature structural & molecular biology 18, 1281-9. PMID: 22037172

6. Fisher, RP. et al. (1992) The Journal of biological chemistry 267, 3358-67. PMID: 1737790

7. Gangelhoff, TA. et al. (2009) Nucleic acids research 37, 3153-64. PMID: 19304746

8. Kasashima, K. et al. (2012) Experimental cell research 318, 2335-43. PMID: 22841477

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Human TFAM polyclonal antibody at 1ug/mL on recombinant Human TFAM (Catalog No. HX990012).

References

Datasheet

Anti-Human TFAM Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

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Anti-Human TFAM Polyclonal Antibody (HX990014)

Overview

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References

Datasheet

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