Human TFE3 Recombinant Protein (N-His) (HB043012)

Name: Human TFE3 Recombinant Protein (N-His)
Brand: abinScience
SKU: HB043012
Price: 211 USD
Availability: InStock

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Vista general

Número de catálogo	HB043012
Descripción	Recombinant Human TFE3 Protein, N-His (HB043012) expressed in E. coli, spanning Asn185-Ser281. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	P19532
Longitud de proteína	Asn185-Ser281
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Homo sapiens (Human)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	12.58 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	TFE3, bHLHe33, BHLHE33, Transcription factor E3, Class E basic helix-loop-helix protein 33
Fondo	Transcription factor E3 (TFE3) is a ~61 kDa protein. Transcription factor that acts as a master regulator of lysosomal biogenesis and immune response. Specifically recognizes and binds E-box sequences (5'-CANNTG-3'); efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFEB or MITF. Involved in the cellular response to amino acid availability by acting downstream of MTOR: in the presence of nutrients, TFE3 phosphorylation by MTOR promotes its inactivation. Upon starvation or lysosomal stress, inhibition of MTOR induces TFE3 dephosphorylation, resulting in transcription factor activity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression, thereby playing a central role in expression of lysosomal genes. 1. Beckmann, H. et al. (1990) Genes & development 4, 167-79. PMID: 2338243 2. Martina, JA. et al. (2014) Science signaling 7, ra9. PMID: 24448649 3. Willett, R. et al. (2017) Nature communications 8, 1580. PMID: 29146937 4. Mathieu, J. et al. (2019) Nature communications 10, 632. PMID: 30733432 5. Lawrence, RE. et al. (2019) Science (New York, N.Y.) 366, 971-977. PMID: 31672913 6. Malik, N. et al. (2023) Science (New York, N.Y.) 380, eabj5559. PMID: 37079666 7. Nardone, C. et al. (2023) Molecular cell 83, 57-73.e9. PMID: 36608670
Nota	For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Recombinant Human TFE3 Protein, N-His

Referencias

Datasheet

Human TFE3 Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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C₁ (Stock Conc.)

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Working Solution

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V₂ (Working Vol.)

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