Anti-Human TFE3 Polyclonal Antibody (HB043014)

Name: Anti-Human TFE3 Polyclonal Antibody
Brand: abinScience
SKU: HB043014
Price: 96 USD
Availability: InStock

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References
Datasheet
SDS

Overview

Catalog No.

HB043014

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human TFE3 (Asn185-Ser281).

Target

TFE3, bHLHe33, BHLHE33, Transcription factor E3, Class E basic helix-loop-helix protein 33

Purification

Purified by antigen affinity column.

Accession

P19532

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Transcription factor E3 (TFE3) is a ~61 kDa protein. Transcription factor that acts as a master regulator of lysosomal biogenesis and immune response. Specifically recognizes and binds E-box sequences (5'-CANNTG-3'); efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFEB or MITF. Involved in the cellular response to amino acid availability by acting downstream of MTOR: in the presence of nutrients, TFE3 phosphorylation by MTOR promotes its inactivation. Upon starvation or lysosomal stress, inhibition of MTOR induces TFE3 dephosphorylation, resulting in transcription factor activity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression, thereby playing a central role in expression of lysosomal genes.

1. Beckmann, H. et al. (1990) Genes & development 4, 167-79. PMID: 2338243

2. Martina, JA. et al. (2014) Science signaling 7, ra9. PMID: 24448649

3. Willett, R. et al. (2017) Nature communications 8, 1580. PMID: 29146937

4. Mathieu, J. et al. (2019) Nature communications 10, 632. PMID: 30733432

5. Lawrence, RE. et al. (2019) Science (New York, N.Y.) 366, 971-977. PMID: 31672913

6. Malik, N. et al. (2023) Science (New York, N.Y.) 380, eabj5559. PMID: 37079666

7. Nardone, C. et al. (2023) Molecular cell 83, 57-73.e9. PMID: 36608670

Note

For research use only.

Images

References

Datasheet

Anti-Human TFE3 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human TFE3 Polyclonal Antibody (HB043014)

Overview

Images

References

Datasheet

SDS

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