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Human TGM1 Recombinant Protein (N-His)
Human TGM1 Recombinant Protein (N-His)
Цена(USD):
Спецификация:
  • 50ug
  • 100ug
  • 1mg
Количество:
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  • Обзор

  • Изображения

  • Ссылки

  • Даташит

  • SDS

Обзор
Каталожный номерHB971012
Описание
Recombinant Human TGM1 Protein, N-His (HB971012) expressed in E. coli, spanning Ser467-Ala817. Purity: >90% by SDS-PAGE.
Highlights
  • ●His-Tagged — N-terminal 6×His tag for IMAC purification.
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессииE. coli
Номер доступаP22735
Длина белкаSer467-Ala817
ПримененияELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
ВидыHomo sapiens (Human)
Характер Recombinant
Уровень эндотоксинов Please contact with the lab for this information.
Чистота >90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса 40.28 kDa
Форма Lyophilized
Буфер хранения Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Реконституция Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.

RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названияKTG, Transglutaminase K, Epidermal TGase, Protein-glutamine gamma-glutamyltransferase K, TGK, TGase K, TGase-1, TGM1, TG(K), Transglutaminase-1
Фон

Protein-glutamine gamma-glutamyltransferase K (TGM1) is a ~89 kDa protein. Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins. Responsible for cross-linking epidermal proteins during formation of the stratum corneum. Involved in cell proliferation.

1. Kim, SY. et al. (1995) The Journal of biological chemistry 270, 18026-35. PMID: 7629111
2. Steinert, PM. et al. (1996) The Journal of biological chemistry 271, 26242-50. PMID: 8824274
3. Zhang, SQ. et al. (2016) International journal of dermatology 55, 201-7. PMID: 26220141
4. Jiang, H. et al. (2010) The Journal of biological chemistry 285, 31634-46. PMID: 20663883
Примечание For research use only.
Изображения
  • Human TGM1 Recombinant Protein (N-His)

    SDS-PAGE

    SDS-PAGE for Recombinant Human TGM1 Protein, N-His

Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Рекомендация

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