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Anti-TGM1 Polyclonal Antibody (HB971014)

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Overview
Catalog No.HB971014
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human TGM1 (Ser467-Ala817).
Target KTG, Transglutaminase K, Epidermal TGase, Protein-glutamine gamma-glutamyltransferase K, TGK, TGase K, TGase-1, TGM1, TG(K), Transglutaminase-1
Purification Purified by antigen affinity column.
Accession P22735
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Protein-glutamine gamma-glutamyltransferase K (TGM1) is a ~89 kDa protein. Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins. Responsible for cross-linking epidermal proteins during formation of the stratum corneum. Involved in cell proliferation.

1. Kim, SY. et al. (1995) The Journal of biological chemistry 270, 18026-35. PMID: 7629111
2. Steinert, PM. et al. (1996) The Journal of biological chemistry 271, 26242-50. PMID: 8824274
3. Zhang, SQ. et al. (2016) International journal of dermatology 55, 201-7. PMID: 26220141
4. Jiang, H. et al. (2010) The Journal of biological chemistry 285, 31634-46. PMID: 20663883
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-TGM1 Polyclonal Antibody

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