中文
English
한국어
日本語
Español
Français
Русский
| カタログ番号 | HB883032 |
|---|---|
| 説明 |
Recombinant Human TIA1 Protein, N-His (HB883032) expressed in E. coli, spanning Asp104-Gly284. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | P31483 |
| タンパク質長 | Asp104-Gly284 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 22.38 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | Cytotoxic granule associated RNA binding protein TIA1, Nucleolysin TIA-1 isoform p40, RNA-binding protein TIA-1, T-cell-restricted intracellular antigen-1, TIA-1, TIA1, p40-TIA-1 |
| 背景 | Cytotoxic granule associated RNA binding protein TIA1 is a ~42 kDa protein. RNA-binding protein involved in the regulation of alternative pre-RNA splicing and mRNA translation by binding to uridine-rich (U-rich) RNA sequences. Binds to U-rich sequences immediately downstream from a 5' splice sites in a uridine-rich small nuclear ribonucleoprotein (U snRNP)-dependent fashion, thereby modulating alternative pre-RNA splicing. Preferably binds to the U-rich IAS1 sequence in a U1 snRNP-dependent manner; this binding is optimal if a 5' splice site is adjacent to IAS1. Activates the use of heterologous 5' splice sites; the activation depends on the intron sequence downstream from the 5' splice site, with a preference for a downstream U-rich sequence. By interacting with SNRPC/U1-C, promotes recruitment and binding of spliceosomal U1 snRNP to 5' splice sites followed by U-rich sequences, thereby facilitating atypical 5' splice site recognition by U1 snRNP. 1. Förch, P. et al. (2000) Molecular cell 6, 1089-98. PMID: 11106748 2. Förch, P. et al. (2002) The EMBO journal 21, 6882-92. PMID: 12486009 3. Izquierdo, JM. et al. (2007) The Journal of biological chemistry 282, 19410-7. PMID: 17488725 4. Dember, LM. et al. (1996) The Journal of biological chemistry 271, 2783-8. PMID: 8576255 7. Zuccato, E. et al. (2004) The Journal of biological chemistry 279, 16980-8. PMID: 14966131 |
| 注意事項 | For research use only |
+86-027-65523339
中国武漢市深敦寺路666号C棟、武漢、430206
