Anti-Human TIA1 Polyclonal Antibody (HB883014)

Name: Anti-Human TIA1 Polyclonal Antibody
Brand: abinScience
SKU: HB883014
Price: 96 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HB883014

Description

Anti-Human TIA1 Polyclonal Antibody (HB883014) is a rabbit polyclonal antibody detecting TIA1 in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human TIA1 (Asp104-Trp181).

Target

TIA1, T-cell-restricted intracellular antigen-1, RNA-binding protein TIA-1, TIA-1, Nucleolysin TIA-1 isoform p40, p40-TIA-1

Purification

Purified by antigen affinity column.

Accession

P31483

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Cytotoxic granule associated RNA binding protein TIA1 is a ~42 kDa protein. RNA-binding protein involved in the regulation of alternative pre-RNA splicing and mRNA translation by binding to uridine-rich (U-rich) RNA sequences. Binds to U-rich sequences immediately downstream from a 5' splice sites in a uridine-rich small nuclear ribonucleoprotein (U snRNP)-dependent fashion, thereby modulating alternative pre-RNA splicing. Preferably binds to the U-rich IAS1 sequence in a U1 snRNP-dependent manner; this binding is optimal if a 5' splice site is adjacent to IAS1. Activates the use of heterologous 5' splice sites; the activation depends on the intron sequence downstream from the 5' splice site, with a preference for a downstream U-rich sequence. By interacting with SNRPC/U1-C, promotes recruitment and binding of spliceosomal U1 snRNP to 5' splice sites followed by U-rich sequences, thereby facilitating atypical 5' splice site recognition by U1 snRNP.

1. Förch, P. et al. (2000) Molecular cell 6, 1089-98. PMID: 11106748

2. Förch, P. et al. (2002) The EMBO journal 21, 6882-92. PMID: 12486009

3. Izquierdo, JM. et al. (2007) The Journal of biological chemistry 282, 19410-7. PMID: 17488725

4. Dember, LM. et al. (1996) The Journal of biological chemistry 271, 2783-8. PMID: 8576255

7. Zuccato, E. et al. (2004) The Journal of biological chemistry 279, 16980-8. PMID: 14966131

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-TIA1 polyclonal antibody at 1μg/ml on various samples.
Lane 1: Hela cell lysate
Lane 2: 293T cell lysate
Lane 3: K562 cell lysate
Lane 4: Mouse brain lysate
Lane 5: Rat brain lysate