Human TIA1 Recombinant Protein (N-His-SUMO & C-Strep) (HB883012)

Name: Human TIA1 Recombinant Protein (N-His-SUMO & C-Strep)
Brand: abinScience
SKU: HB883012
Price: 211 USD
Availability: InStock

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Número de catálogo	HB883012
Descripción	Recombinant Human TIA1 Protein, N-His-SUMO & C-Strep (HB883012) expressed in E. coli, spanning Asp104-Trp181. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	P31483
Longitud de proteína	Asp104-Trp181
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Homo sapiens (Human)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	22.37 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	TIA1, T-cell-restricted intracellular antigen-1, RNA-binding protein TIA-1, TIA-1, Nucleolysin TIA-1 isoform p40, p40-TIA-1
Fondo	Cytotoxic granule associated RNA binding protein TIA1 is a ~42 kDa protein. RNA-binding protein involved in the regulation of alternative pre-RNA splicing and mRNA translation by binding to uridine-rich (U-rich) RNA sequences. Binds to U-rich sequences immediately downstream from a 5' splice sites in a uridine-rich small nuclear ribonucleoprotein (U snRNP)-dependent fashion, thereby modulating alternative pre-RNA splicing. Preferably binds to the U-rich IAS1 sequence in a U1 snRNP-dependent manner; this binding is optimal if a 5' splice site is adjacent to IAS1. Activates the use of heterologous 5' splice sites; the activation depends on the intron sequence downstream from the 5' splice site, with a preference for a downstream U-rich sequence. By interacting with SNRPC/U1-C, promotes recruitment and binding of spliceosomal U1 snRNP to 5' splice sites followed by U-rich sequences, thereby facilitating atypical 5' splice site recognition by U1 snRNP. 1. Förch, P. et al. (2000) Molecular cell 6, 1089-98. PMID: 11106748 2. Förch, P. et al. (2002) The EMBO journal 21, 6882-92. PMID: 12486009 3. Izquierdo, JM. et al. (2007) The Journal of biological chemistry 282, 19410-7. PMID: 17488725 4. Dember, LM. et al. (1996) The Journal of biological chemistry 271, 2783-8. PMID: 8576255 7. Zuccato, E. et al. (2004) The Journal of biological chemistry 279, 16980-8. PMID: 14966131
Nota	For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Recombinant Human TIA1 Protein, N-His-SUMO & C-Strep

Referencias

Datasheet

Human TIA1 Recombinant Protein (N-His-SUMO & C-Strep).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

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Working Solution

C₂ (Working Conc.)

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