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Обзор
Каталожный номерHD613012
Описание
Recombinant Human TP53I3 Protein, N-His (HD613012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% as determined by SDS-PAGE.
Система экспрессииE. coli
Номер доступаQ53FA7
Длина белкаMet1-Gln332
ПримененияELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
ВидыHomo sapiens (Human)
Характер Recombinant
Уровень эндотоксинов Please contact with the lab for this information.
Чистота >90% as determined by SDS-PAGE.
Форма Lyophilized
Буфер хранения Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Реконституция Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Доставка In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названияEC:1.6.5.5, NADPH:quinone reductase PIG3, PIG3, Protein PIG3, Quinone oxidoreductase PIG3, TP53I3, Tumor protein p53-inducible protein 3, p53-induced gene 3 protein
Фон

Quinone oxidoreductase PIG3 is a ~35 kDa protein. NADPH:quinone reductase which expression is induced by p53/TP53 and is involved in both the DNA damage response through homologous recombination repair, and p53-mediated apoptosis. Catalyzes the NADPH-dependent reduction of quinones, exhibiting a low enzymatic activity with beta-naphthoquinones and a strong preference for the ortho-quinone isomer (1,2-beta-naphthoquinone) over the para isomer (1,4-beta-naphthoquinone). Also displays a low reductase activity for non-quinone compounds such as diamine and 2,6-dichloroindophenol (in vitro). The reduction of 1,2-naphthoquinone concurrently generates reactive oxygen species (ROS), which may trigger apoptosis downstream of p53/TP53.

1. Porté, S. et al. (2009) The Journal of biological chemistry 284, 17194-17205. PMID: 19349281
2. Chaudhry, SR. et al. (2021) Cell death discovery 7, 62. PMID: 33782397
Примечание For research use only
Изображения
Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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