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| カタログ番号 | HF892012 |
|---|---|
| 説明 |
Recombinant Human TPPP Protein, N-His (HF892012) expressed in E. coli, spanning Ala8-Thr210. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | O94811 |
| タンパク質長 | Ala8-Thr210 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 24.24 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | 25 kDa brain-specific protein, TPPP/p25, Tubulin polymerization-promoting protein, p24, p25-alpha, TPPP, TPPP1 |
| 背景 | Tubulin polymerization-promoting protein (TPPP) is a ~23 kDa protein. Regulator of microtubule dynamics that plays a key role in myelination by promoting elongation of the myelin sheath. Acts as a microtubule nucleation factor in oligodendrocytes: specifically localizes to the postsynaptic Golgi apparatus region, also named Golgi outpost, and promotes microtubule nucleation, an important step for elongation of the myelin sheath. Required for both uniform polarized growth of distal microtubules as well as directing the branching of proximal processes. Shows magnesium-dependent GTPase activity; the role of the GTPase activity is unclear. In addition to microtubule nucleation activity, also involved in microtubule bundling and stabilization of existing microtubules, thereby maintaining the integrity of the microtubule network. 1. Fu, MM. et al. (2019) Cell 179, 132-146.e14. PMID: 31522887 2. Doi, S. et al. (2021) Cell calcium 96, 102404. PMID: 33831707 3. Zotter, A. et al. (2011) FEBS letters 585, 803-8. PMID: 21316364 4. Zotter, Á. et al. (2011) Biochemistry 50, 9568-78. PMID: 21995432 5. Vincze, O. et al. (2006) Biochemistry 45, 13818-26. PMID: 17105200 6. Hlavanda, E. et al. (2007) The Journal of biological chemistry 282, 29531-9. PMID: 17693641 7. Acevedo, K. et al. (2007) Experimental cell research 313, 4091-106. PMID: 18028908 8. DeBonis, S. et al. (2015) Scientific reports 5, 13242. PMID: 26289831 9. Tokési, N. et al. (2010) The Journal of biological chemistry 285, 17896-906. PMID: 20308065 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human TPPP Protein, N-His
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