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| カタログ番号 | HP992012 |
|---|---|
| 説明 |
Recombinant Human TRAIP Protein, N-His-SUMO (HP992012) expressed in E. coli, spanning Pro2-Asp75. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q9BWF2 |
| タンパク質長 | Pro2-Asp75 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 20.89 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | TRIP, RING finger protein 206, RNF206, TRAF-interacting protein, E3 ubiquitin-protein ligase TRAIP, TRAIP |
| 背景 | E3 ubiquitin-protein ligase TRAIP is a ~53 kDa protein. E3 ubiquitin ligase required to protect genome stability in response to replication stress. Acts as a key regulator of interstrand cross-link repair, which takes place when both strands of duplex DNA are covalently tethered together, thereby blocking replication and transcription. Controls the choice between the two pathways of replication-coupled interstrand-cross-link repair by mediating ubiquitination of MCM7 subunit of the CMG helicase complex. Short ubiquitin chains on MCM7 promote recruitment of DNA glycosylase NEIL3. If the interstrand cross-link cannot be cleaved by NEIL3, the ubiquitin chains continue to grow on MCM7, promoting the unloading of the CMG helicase complex by the VCP/p97 ATPase, enabling the Fanconi anemia DNA repair pathway. 1. Chapard, C. et al. (2014) Journal of cell science 127, 5149-56. PMID: 25335891 2. Harley, ME. et al. (2016) Nature genetics 48, 36-43. PMID: 26595769 3. Hoffmann, S. et al. (2016) The Journal of cell biology 212, 63-75. PMID: 26711499 4. Soo Lee, N. et al. (2016) Nature communications 7, 10463. PMID: 26781088 5. Feng, W. et al. (2016) Cell discovery 2, 16016. PMID: 27462463 6. Sonneville, R. et al. (2019) eLife 8. PMID: 31545170 7. Wallace, HA. et al. (2014) Development (Cambridge, England) 141, 1332-41. PMID: 24553286 8. Zhang, M. et al. (2012) The Journal of experimental medicine 209, 1703-11. PMID: 22945920 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human TRAIP Protein, N-His-SUMO
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