Anti-Human TRAIP Polyclonal Antibody (HP992014)

Name: Anti-Human TRAIP Polyclonal Antibody
Brand: abinScience
SKU: HP992014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HP992014

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human TRAIP (Pro2-Asp75).

Target

TRIP, RING finger protein 206, RNF206, TRAF-interacting protein, E3 ubiquitin-protein ligase TRAIP, TRAIP

Purification

Purified by antigen affinity column.

Accession

Q9BWF2

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

E3 ubiquitin-protein ligase TRAIP is a ~53 kDa protein. E3 ubiquitin ligase required to protect genome stability in response to replication stress. Acts as a key regulator of interstrand cross-link repair, which takes place when both strands of duplex DNA are covalently tethered together, thereby blocking replication and transcription. Controls the choice between the two pathways of replication-coupled interstrand-cross-link repair by mediating ubiquitination of MCM7 subunit of the CMG helicase complex. Short ubiquitin chains on MCM7 promote recruitment of DNA glycosylase NEIL3. If the interstrand cross-link cannot be cleaved by NEIL3, the ubiquitin chains continue to grow on MCM7, promoting the unloading of the CMG helicase complex by the VCP/p97 ATPase, enabling the Fanconi anemia DNA repair pathway.

1. Chapard, C. et al. (2014) Journal of cell science 127, 5149-56. PMID: 25335891

2. Harley, ME. et al. (2016) Nature genetics 48, 36-43. PMID: 26595769

3. Hoffmann, S. et al. (2016) The Journal of cell biology 212, 63-75. PMID: 26711499

4. Soo Lee, N. et al. (2016) Nature communications 7, 10463. PMID: 26781088

5. Feng, W. et al. (2016) Cell discovery 2, 16016. PMID: 27462463

6. Sonneville, R. et al. (2019) eLife 8. PMID: 31545170

7. Wallace, HA. et al. (2014) Development (Cambridge, England) 141, 1332-41. PMID: 24553286

8. Zhang, M. et al. (2012) The Journal of experimental medicine 209, 1703-11. PMID: 22945920

Note

For research use only.

Images

References

Datasheet

Anti-Human TRAIP Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Assay Kits

Anti-Human TRAIP Polyclonal Antibody (HP992014)

Overview

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References

Datasheet

SDS

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