Human TRIM14 Recombinant Protein (N-His) (HC308012)

Name: Human TRIM14 Recombinant Protein (N-His)
Brand: abinScience
SKU: HC308012
Price: 211 USD
Availability: InStock

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50ug
100ug
1mg

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Обзор

Каталожный номер	HC308012
Описание	Recombinant Human TRIM14 Protein, N-His (HC308012) expressed in E. coli, spanning Ser254-Pro442. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессии	E. coli
Номер доступа	Q14142
Длина белка	Ser254-Pro442
Применения	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды	Homo sapiens (Human)
Характер	Recombinant
Уровень эндотоксинов	Please contact with the lab for this information.
Чистота	>90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса	23.77 kDa
Форма	Lyophilized
Буфер хранения	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия	KIAA0129, Tripartite motif-containing protein 14, TRIM14
Фон	Tripartite motif-containing protein 14 (TRIM14) is a ~49 kDa protein. Plays an essential role in the innate immune defense against viruses and bacteria. Promotes the 'Lys-48'-linked ubiquitination and subsequent degradation of hepatitis C virus NS5A leading to the inhibition of viral replication. Also plays a role in the inhibition of ebolavirus infection by enhancing IFN-beta and NF-kappa-B activation after binding to the viral protein NP. Facilitates the type I IFN response by interacting with MAVS at the outer mitochondria membrane and thereby recruiting NF-kappa-B essential modulator IKBKG/NEMO to the MAVS signalosome, leading to the activation of both the IFN regulatory factor 3/IRF3 and NF-kappa-B pathways. Positively regulates the CGAS-induced type I interferon signaling pathway by stabilizing CGAS and inhibiting its autophagic degradation. 1. Tan, G. et al. (2018) Frontiers in immunology 9, 1872. PMID: 30150992 2. Hoffpauir, CT. et al. (2020) Journal of immunology (Baltimore, Md. : 1950) 205, 153-167. PMID: 32404352 3. Wang, S. et al. (2016) Scientific reports 6, 32336. PMID: 27578425 4. Kuroda, M. et al. (2023) The Journal of infectious diseases 228, S514-S521. PMID: 37562033 5. Zhou, Z. et al. (2014) Proceedings of the National Academy of Sciences of the United States of America 111, E245-54. PMID: 24379373 6. Chen, M. et al. (2016) Molecular cell 64, 105-119. PMID: 27666593 7. Liu, D. et al. (2022) Proceedings of the National Academy of Sciences of the United States of America 119. PMID: 35145029
Примечание	For research use only.

Изображения

SDS-PAGE

SDS-PAGE for Recombinant Human TRIM14 Protein, N-His

Ссылки

Даташит

Human TRIM14 Recombinant Protein (N-His).pdf

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Formula

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C₁ (Stock Conc.)

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V₂ (Working Vol.)

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