Human TrkB Recombinant Protein (N-His) (HS892012)

Name: Human TrkB Recombinant Protein (N-His)
Brand: abinScience
SKU: HS892012
Price: 211 USD
Availability: InStock

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Aperçu

Numéro de catalogue	HS892012
Description	Recombinant Human TrkB /NTRK2 Protein, N-His (HS892012) expressed in E. coli, spanning Cys32-His430. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression	E. coli
Numéro d'accès	Q16620
Longueur de protéine	Cys32-His430
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces	Homo sapiens (Human)
Nature	Recombinant
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>90% as determined by SDS-PAGE.
Masse moléculaire prédite	46.5 kDa
Forme	Lyophilized
Tampon de stockage	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	Neurotrophic tyrosine kinase receptor type 2, TrkB tyrosine kinase, GP145-TrkB, TRKB, NTRK2, Tropomyosin-related kinase B, BDNF/NT-3 growth factors receptor, Trk-B
Contexte	BDNF/NT-3 growth factors receptor (TrkB/NTRK2) is a ~91 kDa protein. Receptor tyrosine kinase involved in the development and the maturation of the central and the peripheral nervous systems through regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity. Receptor for BDNF/brain-derived neurotrophic factor and NTF4/neurotrophin-4. Alternatively can also bind NTF3/neurotrophin-3 which is less efficient in activating the receptor but regulates neuron survival through NTRK2. Upon ligand-binding, undergoes homodimerization, autophosphorylation and activation. Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades. 1. Yeo, GS. et al. (2004) Nature neuroscience 7, 1187-9. PMID: 15494731 2. Haniu, M. et al. (1995) Archives of biochemistry and biophysics 322, 256-64. PMID: 7574684
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human TrkB /NTRK2 Protein, N-His

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Human TrkB Recombinant Protein (N-His).pdf

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Formula

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Stock Solution

C₁ (Stock Conc.)

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