Anti-Human TrkB Reference Antibody (ZEB85, RUO) (HS892016)

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Overview

Catalog No.	HS892016
Description	Anti-Human TrkB /NTRK2 (ZEB85) (HS892016) is a research-grade recombinant antibody targeting Neurotrophic tyrosine kinase receptor type 2. Produced in mammalian cells with native-like glycosylation. Highlights ●Research Grade — For PK/PD studies, assay development, and ADA research. ●Native Glycosylation — Mammalian expression ensures native-like patterns.
Species reactivity	Human
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Host species	Human
Isotype	scFv-Fc
Clone ID	ZEB85
Expression system	Mammalian cells
Clonality	Monoclonal
Target	Neurotrophic tyrosine kinase receptor type 2, TrkB tyrosine kinase, GP145-TrkB, TRKB, NTRK2, Tropomyosin-related kinase B, BDNF/NT-3 growth factors receptor, Trk-B
Endotoxin level	Please contact the lab for this information.
Purity	>95% purity as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	Q16620
Form	Liquid
Storage buffer	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	ZEB85
Background	BDNF/NT-3 growth factors receptor (TrkB/NTRK2) is a ~91 kDa protein. Receptor tyrosine kinase involved in the development and the maturation of the central and the peripheral nervous systems through regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity. Receptor for BDNF/brain-derived neurotrophic factor and NTF4/neurotrophin-4. Alternatively can also bind NTF3/neurotrophin-3 which is less efficient in activating the receptor but regulates neuron survival through NTRK2. Upon ligand-binding, undergoes homodimerization, autophosphorylation and activation. Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades. 1. Yeo, GS. et al. (2004) Nature neuroscience 7, 1187-9. PMID: 15494731 2. Haniu, M. et al. (1995) Archives of biochemistry and biophysics 322, 256-64. PMID: 7574684
Note	For research use only. Not suitable for clinical or therapeutic use.

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SDS-PAGE

SDS-PAGE for Research Grade Anti-Human TrkB /NTRK2 (ZEB85)
Flow cytometry

Flow-cytometry using anti-human TrkB antibody. SH-SY5Y cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human TrkB monoclonal antibody (Catalog HS892016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Bioactivity

Detects recombinant NTRK2 (Catalog No: HS892011) in indirect ELISA.

References

Datasheet

Anti-Human TrkB Reference Antibody (ZEB85, RUO).pdf

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Anti-Human TrkB Reference Antibody (ZEB85, RUO) (HS892016)

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