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| カタログ番号 | HA519012 |
|---|---|
| 説明 |
Recombinant Human TRPM2 Protein, N-His (HA519012) expressed in E. coli, spanning Lys140-Arg358. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | O94759 |
| タンパク質長 | Lys140-Arg358 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 27.04 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | Transient receptor potential cation channel subfamily M member 2, Estrogen-responsive element-associated gene 1 protein, Long transient receptor potential channel 2, LTrpC-2, LTrpC2, Transient receptor potential channel 7, TrpC7, Transient receptor potential melastatin 2, TRPM2, EREG1, KNP3, LTRPC2, TRPC7 |
| 背景 | Transient receptor potential cation channel subfamily M member 2 is a ~171 kDa protein. Nonselective, voltage-independent cation channel that mediates Na(+) and Ca(2+) influx, leading to increased cytoplasmic Ca(2+) levels. Functions as a ligand-gated ion channel, gated by intracellular adenosine diphosphate ribose (ADP-ribose), Ca(2+), warm temperature, and oxidative stress. The precise physiological activators are under debate; the true, physiological activators may be ADP-ribose and ADP-ribose-2'-phosphate. Binding of ADP-ribose to the cytoplasmic Nudix domain causes a conformation change; the channel is primed but still requires Ca(2+) binding to trigger channel opening. Extracellular Ca(2+) passes through the channel and increases channel activity. 1. Perraud, AL. et al. (2001) Nature 411, 595-9. PMID: 11385575 2. Sano, Y. et al. (2001) Science (New York, N.Y.) 293, 1327-30. PMID: 11509734 3. Hara, Y. et al. (2002) Molecular cell 9, 163-73. PMID: 11804595 4. Zhang, W. et al. (2003) The Journal of biological chemistry 278, 16222-9. PMID: 12594222 5. Perraud, AL. et al. (2005) The Journal of biological chemistry 280, 6138-48. PMID: 15561722 6. Togashi, K. et al. (2006) The EMBO journal 25, 1804-15. PMID: 16601673 7. Csanády, L. et al. (2009) The Journal of general physiology 133, 189-203. PMID: 19171771 8. Yang, W. et al. (2010) The Journal of biological chemistry 285, 30411-8. PMID: 20660597 9. Kühn, FJ. et al. (2015) Scientific reports 5, 8032. PMID: 25620041 10. Li, F. et al. (2016) Journal of cell science 129, 2016-29. PMID: 27068538 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human TRPM2 Protein, N-His
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