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| カタログ番号 | HP132012 |
|---|---|
| 説明 |
Recombinant Human TUT4 Protein, N-His (HP132012) expressed in E. coli, spanning Glu246-Asp370. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q5TAX3 |
| タンパク質長 | Glu246-Asp370 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 16.71 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | TUTase 4, KIAA0191, TUT4, ZCCHC11, Terminal uridylyltransferase 4, Zinc finger CCHC domain-containing protein 11 |
| 背景 | Terminal uridylyltransferase 4 (TUT4) is a ~185 kDa protein. Uridylyltransferase that mediates the terminal uridylation of mRNAs with short (less than 25 nucleotides) poly(A) tails, hence facilitating global mRNA decay. Essential for both oocyte maturation and fertility. Through 3' terminal uridylation of mRNA, sculpts, with TUT7, the maternal transcriptome by eliminating transcripts during oocyte growth. Involved in microRNA (miRNA)-induced gene silencing through uridylation of deadenylated miRNA targets. Also functions as an integral regulator of microRNA biogenesis using 3 different uridylation mechanisms. 1. Lim, J. et al. (2014) Cell 159, 1365-76. PMID: 25480299 2. Yamashita, S. et al. (2019) Nature communications 10, 1960. PMID: 31036859 3. Kim, B. et al. (2015) The EMBO journal 34, 1801-15. PMID: 25979828 4. Heo, I. et al. (2009) Cell 138, 696-708. PMID: 19703396 5. Minoda, Y. et al. (2006) Biochemical and biophysical research communications 344, 1023-30. PMID: 16643855 6. Mullen, TE. et al. (2008) Genes & development 22, 50-65. PMID: 18172165 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human TUT4 Protein, N-His
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