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Anti-Human TUT4 Polyclonal Antibody (HP132014)

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Overview
Catalog No.HP132014
Description
Anti-Human TUT4 Polyclonal Antibody (HP132014) is a rabbit polyclonal antibody detecting TUT4 in ELISA, IHC, WB. Suitable for Human and Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human TUT4 (Glu246-Asp370).
Target TUTase 4, KIAA0191, TUT4, ZCCHC11, Terminal uridylyltransferase 4, Zinc finger CCHC domain-containing protein 11
Purification Purified by antigen affinity column.
Accession Q5TAX3
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Terminal uridylyltransferase 4 (TUT4) is a ~185 kDa protein. Uridylyltransferase that mediates the terminal uridylation of mRNAs with short (less than 25 nucleotides) poly(A) tails, hence facilitating global mRNA decay. Essential for both oocyte maturation and fertility. Through 3' terminal uridylation of mRNA, sculpts, with TUT7, the maternal transcriptome by eliminating transcripts during oocyte growth. Involved in microRNA (miRNA)-induced gene silencing through uridylation of deadenylated miRNA targets. Also functions as an integral regulator of microRNA biogenesis using 3 different uridylation mechanisms.

1. Lim, J. et al. (2014) Cell 159, 1365-76. PMID: 25480299
2. Yamashita, S. et al. (2019) Nature communications 10, 1960. PMID: 31036859
3. Kim, B. et al. (2015) The EMBO journal 34, 1801-15. PMID: 25979828
4. Heo, I. et al. (2009) Cell 138, 696-708. PMID: 19703396
5. Minoda, Y. et al. (2006) Biochemical and biophysical research communications 344, 1023-30. PMID: 16643855
6. Mullen, TE. et al. (2008) Genes & development 22, 50-65. PMID: 18172165
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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Anti-Human TUT4 Polyclonal Antibody

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