ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces
Homo sapiens (Human)
Nature
Recombinant
Niveau d'endotoxines
Please contact with the lab for this information.
Pureté
>90% as determined by SDS-PAGE.
Masse moléculaire prédite
31.03 kDa
Forme
Lyophilized
Tampon de stockage
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs
PEO1, Twinkle mtDNA helicase, Progressive external ophthalmoplegia 1 protein, T7-like mitochondrial DNA helicase, TWNK, T7 gp4-like protein with intramitochondrial nucleoid localization, C10orf2, Twinkle protein, mitochondrial
Contexte
Twinkle mtDNA helicase (TWNK) is a ~77 kDa protein. Mitochondrial helicase involved in mtDNA replication and repair. Might have a role in mtDNA repair. Has DNA strand separation activity needed to form a processive replication fork for leading strand synthesis which is catalyzed by the formation of a replisome complex with POLG and mtSDB. Preferentially unwinds DNA substrates with pre-existing 5'-and 3'- single-stranded tails but is also active on a 5'- flap substrate. Can dissociate the invading strand of immobile or mobile D-loop DNA structures irrespective of the single strand polarity of the third strand.
1. Korhonen, JA. et al. (2003) The Journal of biological chemistry 278, 48627-32. PMID: 12975372
2. Korhonen, JA. et al. (2004) The EMBO journal 23, 2423-9. PMID: 15167897
3. Ziebarth, TD. et al. (2007) Journal of molecular biology 367, 1382-91. PMID: 17324440
4. Farge, G. et al. (2008) Nucleic acids research 36, 393-403. PMID: 18039713
5. Goffart, S. et al. (2009) Human molecular genetics 18, 328-40. PMID: 18971204
6. Fernández-Millán, P. et al. (2015) Nucleic acids research 43, 4284-95. PMID: 25824949
7. Sen, D. et al. (2016) Nucleic acids research 44, 4200-10. PMID: 26887820
8. Khan, I. et al. (2016) The Journal of biological chemistry 291, 14324-14339. PMID: 27226550
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Recombinant Human TWNK Protein, N-His
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