Anti-TWNK Polyclonal Antibody (HP123014)

Name: Anti-TWNK Polyclonal Antibody
Brand: abinScience
SKU: HP123014
Price: 168 USD
Availability: InStock

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Datasheet
SDS

Overview

Catalog No.

HP123014

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human TWNK (Val384-Lys635).

Target

PEO1, Twinkle mtDNA helicase, Progressive external ophthalmoplegia 1 protein, T7-like mitochondrial DNA helicase, TWNK, T7 gp4-like protein with intramitochondrial nucleoid localization, C10orf2, Twinkle protein, mitochondrial

Purification

Purified by antigen affinity column.

Accession

Q96RR1

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Twinkle mtDNA helicase (TWNK) is a ~77 kDa protein. Mitochondrial helicase involved in mtDNA replication and repair. Might have a role in mtDNA repair. Has DNA strand separation activity needed to form a processive replication fork for leading strand synthesis which is catalyzed by the formation of a replisome complex with POLG and mtSDB. Preferentially unwinds DNA substrates with pre-existing 5'-and 3'- single-stranded tails but is also active on a 5'- flap substrate. Can dissociate the invading strand of immobile or mobile D-loop DNA structures irrespective of the single strand polarity of the third strand.

1. Korhonen, JA. et al. (2003) The Journal of biological chemistry 278, 48627-32. PMID: 12975372

2. Korhonen, JA. et al. (2004) The EMBO journal 23, 2423-9. PMID: 15167897

3. Ziebarth, TD. et al. (2007) Journal of molecular biology 367, 1382-91. PMID: 17324440

4. Farge, G. et al. (2008) Nucleic acids research 36, 393-403. PMID: 18039713

5. Goffart, S. et al. (2009) Human molecular genetics 18, 328-40. PMID: 18971204

6. Fernández-Millán, P. et al. (2015) Nucleic acids research 43, 4284-95. PMID: 25824949

7. Sen, D. et al. (2016) Nucleic acids research 44, 4200-10. PMID: 26887820

8. Khan, I. et al. (2016) The Journal of biological chemistry 291, 14324-14339. PMID: 27226550

Note

For research use only.

Images

References

Datasheet

Anti-TWNK Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-TWNK Polyclonal Antibody (HP123014)

Overview

Images

References

Datasheet

SDS

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