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Human UBE2G2 Recombinant Protein (N-His) (HD110012)

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概要
カタログ番号HD110012
説明
Recombinant Human UBE2G2 Protein, N-His (HD110012) expressed in E. coli, spanning Met1-Leu165. Purity: >90% by SDS-PAGE.
Highlights
  • ●His-Tagged — N-terminal 6×His tag for IMAC purification.
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% purity verified by SDS-PAGE.
発現システムE. coli
アクセッション番号P60604
タンパク質長Met1-Leu165
アプリケーションELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
種Homo sapiens (Human)
性質 Recombinant
エンドトキシンレベル Please contact with the lab for this information.
純度 >90% as determined by SDS-PAGE.
予測分子量 20.73 kDa
形態 Lyophilized
保存バッファー Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

再構成 Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
発送 In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
安定性と保存 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
別名E2 ubiquitin-conjugating enzyme G2, EC:2.3.2.23, UBC7, UBE2G2, Ubiquitin carrier protein G2, Ubiquitin-conjugating enzyme E2 G2, Ubiquitin-protein ligase G2
背景

Ubiquitin-conjugating enzyme E2 G2 is a ~18 kDa protein. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Involved in endoplasmic reticulum-associated degradation (ERAD). Required for sterol-induced ubiquitination of 3-hydroxy-3-methylglutaryl coenzyme A reductase and its subsequent proteasomal degradation.

1. David, Y. et al. (2010) The Journal of biological chemistry 285, 8595-604. PMID: 20061386
2. Sato, T. et al. (2012) Molecular cell 47, 99-110. PMID: 22607976
3. Jo, Y. et al. (2013) Molecular biology of the cell 24, 169-83. PMID: 23223569
注意事項 For research use only
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参考文献
Formula
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