Human UBE2S Recombinant Protein (N-His) (HS820012)

Name: Human UBE2S Recombinant Protein (N-His)
Brand: abinScience
SKU: HS820012
Price: 211 USD
Availability: InStock

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Vista general

Número de catálogo	HS820012
Descripción	Recombinant Human UBE2S Protein, N-His (HS820012) expressed in E. coli, spanning Met1-Ala209. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	Q16763
Longitud de proteína	Met1-Ala209
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Homo sapiens (Human)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	24.38 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	Ubiquitin-protein ligase S, E2EPF, Ubiquitin-conjugating enzyme E2 S, E2-EPF, Ubiquitin-conjugating enzyme E2-EPF5, Ubiquitin-conjugating enzyme E2-24 kDa, E2 ubiquitin-conjugating enzyme S, Ubiquitin carrier protein S, UBE2S
Fondo	Ubiquitin-conjugating enzyme E2 S (UBE2S) is a ~23 kDa protein. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes 'Lys-11'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by specifically elongating 'Lys-11'-linked polyubiquitin chains initiated by the E2 enzyme UBE2C/UBCH10 on APC/C substrates, enhancing the degradation of APC/C substrates by the proteasome and promoting mitotic exit. Also acts by elongating ubiquitin chains initiated by the E2 enzyme UBE2D1/UBCH5 in vitro; it is however unclear whether UBE2D1/UBCH5 acts as an E2 enzyme for the APC/C in vivo. 1. Garnett, MJ. et al. (2009) Nature cell biology 11, 1363-9. PMID: 19820702 2. Williamson, A. et al. (2009) Proceedings of the National Academy of Sciences of the United States of America 106, 18213-8. PMID: 19822757 3. Sheng, Y. et al. (2012) Molecular & cellular proteomics : MCP 11, 329-41. PMID: 22496338 4. Brown, NG. et al. (2016) Cell 165, 1440-1453. PMID: 27259151 5. Kuang, P. et al. (2016) Scientific reports 6, 37441. PMID: 27910872 7. David, Y. et al. (2010) The Journal of biological chemistry 285, 8595-604. PMID: 20061386 8. Bremm, A. et al. (2010) Nature structural & molecular biology 17, 939-47. PMID: 20622874
Nota	For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Recombinant Human UBE2S Protein, N-His

Referencias

UBE2S interacting with TRIM21 mediates the K11-linked ubiquitination of LPP to promote the lymphatic metastasis of bladder cancer

Datasheet

Human UBE2S Recombinant Protein (N-His).pdf

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Formula

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Stock Solution

C₁ (Stock Conc.)

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Working Solution

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V₂ (Working Vol.)

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