Anti-UBE2S Polyclonal Antibody (HS820014)

Name: Anti-UBE2S Polyclonal Antibody
Brand: abinScience
SKU: HS820014
Price: 168 USD
Availability: InStock

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Datasheet
SDS

Overview

Catalog No.

HS820014

Description

Anti-UBE2S Polyclonal Antibody (HS820014) is a rabbit polyclonal antibody detecting UBE2S in ELISA, IHC, WB. Suitable for Bovine, Human, Mouse, Rat, and Xenopus laevis.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human UBE2S (Met1-Ala209).

Target

Ubiquitin-protein ligase S, E2EPF, Ubiquitin-conjugating enzyme E2 S, E2-EPF, Ubiquitin-conjugating enzyme E2-EPF5, Ubiquitin-conjugating enzyme E2-24 kDa, E2 ubiquitin-conjugating enzyme S, Ubiquitin carrier protein S, UBE2S

Purification

Purified by antigen affinity column.

Accession

Q16763

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Ubiquitin-conjugating enzyme E2 S (UBE2S) is a ~23 kDa protein. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes 'Lys-11'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by specifically elongating 'Lys-11'-linked polyubiquitin chains initiated by the E2 enzyme UBE2C/UBCH10 on APC/C substrates, enhancing the degradation of APC/C substrates by the proteasome and promoting mitotic exit. Also acts by elongating ubiquitin chains initiated by the E2 enzyme UBE2D1/UBCH5 in vitro; it is however unclear whether UBE2D1/UBCH5 acts as an E2 enzyme for the APC/C in vivo.

1. Garnett, MJ. et al. (2009) Nature cell biology 11, 1363-9. PMID: 19820702

2. Williamson, A. et al. (2009) Proceedings of the National Academy of Sciences of the United States of America 106, 18213-8. PMID: 19822757

3. Sheng, Y. et al. (2012) Molecular & cellular proteomics : MCP 11, 329-41. PMID: 22496338

4. Brown, NG. et al. (2016) Cell 165, 1440-1453. PMID: 27259151

5. Kuang, P. et al. (2016) Scientific reports 6, 37441. PMID: 27910872

7. David, Y. et al. (2010) The Journal of biological chemistry 285, 8595-604. PMID: 20061386

8. Bremm, A. et al. (2010) Nature structural & molecular biology 17, 939-47. PMID: 20622874

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-UBE2S polyclonal antibody at 1ug/mL on recombinant UBE2S (Catalog No. HS820012).

References

Datasheet

Anti-UBE2S Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-UBE2S Polyclonal Antibody (HS820014)

Overview

Images

References

Datasheet

SDS

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