Human UBE2T Recombinant Protein (N-His) (HP958012)

Name: Human UBE2T Recombinant Protein (N-His)
Brand: abinScience
SKU: HP958012
Price: 211 USD
Availability: InStock

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100ug
1mg

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SDS

Vista general

Número de catálogo	HP958012
Descripción	Recombinant Human UBE2T Protein, N-His (HP958012) expressed in E. coli, spanning Met1-Arg152. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	Q9NPD8
Longitud de proteína	Met1-Arg152
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Homo sapiens (Human)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	19.57 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	Ubiquitin-protein ligase T, Ubiquitin carrier protein T, UBE2T, Cell proliferation-inducing gene 50 protein, Ubiquitin-conjugating enzyme E2 T, E2 ubiquitin-conjugating enzyme T
Fondo	Ubiquitin-conjugating enzyme E2 T (UBE2T) is a ~22 kDa protein. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in mitomycin-C (MMC)-induced DNA repair. Acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway. Also mediates monoubiquitination of FANCL and FANCI. 1. Machida, YJ. et al. (2006) Molecular cell 23, 589-96. PMID: 16916645 2. Alpi, A. et al. (2007) Molecular and cellular biology 27, 8421-30. PMID: 17938197 3. Alpi, AF. et al. (2008) Molecular cell 32, 767-77. PMID: 19111657 4. Longerich, S. et al. (2009) The Journal of biological chemistry 284, 23182-6. PMID: 19589784 5. Morreale, FE. et al. (2017) Journal of medicinal chemistry 60, 4093-4098. PMID: 28437106 6. Ueki, T. et al. (2009) Cancer research 69, 8752-60. PMID: 19887602 7. David, Y. et al. (2010) The Journal of biological chemistry 285, 8595-604. PMID: 20061386
Nota	For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Recombinant Human UBE2T Protein, N-His

Referencias

Datasheet

Human UBE2T Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Mass

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Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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