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Anti-Human UBE2T Polyclonal Antibody (HP958014)

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Overview
Catalog No.HP958014
Description
Anti-Human UBE2T Polyclonal Antibody (HP958014) is a rabbit polyclonal antibody detecting UBE2T in ELISA, IHC, WB. Suitable for Bovine, Human, and Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human UBE2T (Met1-Arg152).
Target Ubiquitin-protein ligase T, Ubiquitin carrier protein T, UBE2T, Cell proliferation-inducing gene 50 protein, Ubiquitin-conjugating enzyme E2 T, E2 ubiquitin-conjugating enzyme T
Purification Purified by antigen affinity column.
Accession Q9NPD8
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Ubiquitin-conjugating enzyme E2 T (UBE2T) is a ~22 kDa protein. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in mitomycin-C (MMC)-induced DNA repair. Acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway. Also mediates monoubiquitination of FANCL and FANCI.

1. Machida, YJ. et al. (2006) Molecular cell 23, 589-96. PMID: 16916645
2. Alpi, A. et al. (2007) Molecular and cellular biology 27, 8421-30. PMID: 17938197
3. Alpi, AF. et al. (2008) Molecular cell 32, 767-77. PMID: 19111657
4. Longerich, S. et al. (2009) The Journal of biological chemistry 284, 23182-6. PMID: 19589784
5. Morreale, FE. et al. (2017) Journal of medicinal chemistry 60, 4093-4098. PMID: 28437106
6. Ueki, T. et al. (2009) Cancer research 69, 8752-60. PMID: 19887602
7. David, Y. et al. (2010) The Journal of biological chemistry 285, 8595-604. PMID: 20061386
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-Human UBE2T Polyclonal Antibody

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