Human UBE2T Recombinant Protein (N-His) (HP958012)

Name: Human UBE2T Recombinant Protein (N-His)
Brand: abinScience
SKU: HP958012
Price: 211 USD
Availability: InStock

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Aperçu

Numéro de catalogue	HP958012
Description	Recombinant Human UBE2T Protein, N-His (HP958012) expressed in E. coli, spanning Met1-Arg152. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression	E. coli
Numéro d'accès	Q9NPD8
Longueur de protéine	Met1-Arg152
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces	Homo sapiens (Human)
Nature	Recombinant
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>90% as determined by SDS-PAGE.
Masse moléculaire prédite	19.57 kDa
Forme	Lyophilized
Tampon de stockage	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	Ubiquitin-protein ligase T, Ubiquitin carrier protein T, UBE2T, Cell proliferation-inducing gene 50 protein, Ubiquitin-conjugating enzyme E2 T, E2 ubiquitin-conjugating enzyme T
Contexte	Ubiquitin-conjugating enzyme E2 T (UBE2T) is a ~22 kDa protein. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in mitomycin-C (MMC)-induced DNA repair. Acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway. Also mediates monoubiquitination of FANCL and FANCI. 1. Machida, YJ. et al. (2006) Molecular cell 23, 589-96. PMID: 16916645 2. Alpi, A. et al. (2007) Molecular and cellular biology 27, 8421-30. PMID: 17938197 3. Alpi, AF. et al. (2008) Molecular cell 32, 767-77. PMID: 19111657 4. Longerich, S. et al. (2009) The Journal of biological chemistry 284, 23182-6. PMID: 19589784 5. Morreale, FE. et al. (2017) Journal of medicinal chemistry 60, 4093-4098. PMID: 28437106 6. Ueki, T. et al. (2009) Cancer research 69, 8752-60. PMID: 19887602 7. David, Y. et al. (2010) The Journal of biological chemistry 285, 8595-604. PMID: 20061386
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human UBE2T Protein, N-His

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Human UBE2T Recombinant Protein (N-His).pdf

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Formula

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