Human UBQLN4/Ubiquilin-4 Recombinant Protein (N-GST & C-His) (HP715012)

Name: Human UBQLN4/Ubiquilin-4 Recombinant Protein (N-GST & C-His)
Brand: abinScience
SKU: HP715012
Price: 211 USD
Availability: InStock

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Número de catálogo	HP715012
Descripción	Recombinant Human UBQLN4/Ubiquilin-4 Protein, N-GST & C-His (HP715012) expressed in E. coli, spanning Pro12-Lys83. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	Q9NRR5
Longitud de proteína	Pro12-Lys83
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Homo sapiens (Human)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	36.38 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	A1Up, Connexin43-interacting protein of 75 kDa, CIP75, C1orf6, UBIN, Ataxin-1 ubiquitin-like-interacting protein A1U, Ataxin-1 interacting ubiquitin-like protein, UBQLN4, Ubiquilin-4
Fondo	Ubiquilin-4 (UBQLN4) is a ~63 kDa protein. Regulator of protein degradation that mediates the proteasomal targeting of misfolded, mislocalized or accumulated proteins. Acts by binding polyubiquitin chains of target proteins via its UBA domain and by interacting with subunits of the proteasome via its ubiquitin-like domain. Key regulator of DNA repair that represses homologous recombination repair: in response to DNA damage, recruited to sites of DNA damage following phosphorylation by ATM and acts by binding and removing ubiquitinated MRE11 from damaged chromatin, leading to MRE11 degradation by the proteasome. MRE11 degradation prevents homologous recombination repair, redirecting double-strand break repair toward non-homologous end joining (NHEJ). Specifically recognizes and binds mislocalized transmembrane-containing proteins and targets them to proteasomal degradation. 1. Riley, BE. et al. (2004) The Journal of biological chemistry 279, 42290-301. PMID: 15280365 2. Suzuki, R. et al. (2016) EMBO reports 17, 842-57. PMID: 27113755 3. Hirayama, S. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, E4199-E4208. PMID: 29666234 4. Jachimowicz, RD. et al. (2019) Cell 176, 505-519.e22. PMID: 30612738 5. Lee, DY. et al. (2013) EMBO reports 14, 373-81. PMID: 23459205
Nota	For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Recombinant Human UBQLN4/Ubiquilin-4 Protein, N-GST & C-His

Referencias

Datasheet

Human UBQLN4/Ubiquilin-4 Recombinant Protein (N-GST & C-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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