Human UBQLN4/Ubiquilin-4 Recombinant Protein (N-GST & C-His) (HP715012)

Name: Human UBQLN4/Ubiquilin-4 Recombinant Protein (N-GST & C-His)
Brand: abinScience
SKU: HP715012
Price: 211 USD
Availability: InStock

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Aperçu

Numéro de catalogue	HP715012
Description	Recombinant Human UBQLN4/Ubiquilin-4 Protein, N-GST & C-His (HP715012) expressed in E. coli, spanning Pro12-Lys83. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression	E. coli
Numéro d'accès	Q9NRR5
Longueur de protéine	Pro12-Lys83
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces	Homo sapiens (Human)
Nature	Recombinant
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>90% as determined by SDS-PAGE.
Masse moléculaire prédite	36.38 kDa
Forme	Lyophilized
Tampon de stockage	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	A1Up, Connexin43-interacting protein of 75 kDa, CIP75, C1orf6, UBIN, Ataxin-1 ubiquitin-like-interacting protein A1U, Ataxin-1 interacting ubiquitin-like protein, UBQLN4, Ubiquilin-4
Contexte	Ubiquilin-4 (UBQLN4) is a ~63 kDa protein. Regulator of protein degradation that mediates the proteasomal targeting of misfolded, mislocalized or accumulated proteins. Acts by binding polyubiquitin chains of target proteins via its UBA domain and by interacting with subunits of the proteasome via its ubiquitin-like domain. Key regulator of DNA repair that represses homologous recombination repair: in response to DNA damage, recruited to sites of DNA damage following phosphorylation by ATM and acts by binding and removing ubiquitinated MRE11 from damaged chromatin, leading to MRE11 degradation by the proteasome. MRE11 degradation prevents homologous recombination repair, redirecting double-strand break repair toward non-homologous end joining (NHEJ). Specifically recognizes and binds mislocalized transmembrane-containing proteins and targets them to proteasomal degradation. 1. Riley, BE. et al. (2004) The Journal of biological chemistry 279, 42290-301. PMID: 15280365 2. Suzuki, R. et al. (2016) EMBO reports 17, 842-57. PMID: 27113755 3. Hirayama, S. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, E4199-E4208. PMID: 29666234 4. Jachimowicz, RD. et al. (2019) Cell 176, 505-519.e22. PMID: 30612738 5. Lee, DY. et al. (2013) EMBO reports 14, 373-81. PMID: 23459205
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human UBQLN4/Ubiquilin-4 Protein, N-GST & C-His

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Human UBQLN4/Ubiquilin-4 Recombinant Protein (N-GST & C-His).pdf

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