Human UBQLN4/Ubiquilin-4 Recombinant Protein (N-GST & C-His) (HP715012)

Name: Human UBQLN4/Ubiquilin-4 Recombinant Protein (N-GST & C-His)
Brand: abinScience
SKU: HP715012
Price: 211 USD
Availability: InStock

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100ug
1mg

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Каталожный номер	HP715012
Описание	Recombinant Human UBQLN4/Ubiquilin-4 Protein, N-GST & C-His (HP715012) expressed in E. coli, spanning Pro12-Lys83. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессии	E. coli
Номер доступа	Q9NRR5
Длина белка	Pro12-Lys83
Применения	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды	Homo sapiens (Human)
Характер	Recombinant
Уровень эндотоксинов	Please contact with the lab for this information.
Чистота	>90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса	36.38 kDa
Форма	Lyophilized
Буфер хранения	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия	A1Up, Connexin43-interacting protein of 75 kDa, CIP75, C1orf6, UBIN, Ataxin-1 ubiquitin-like-interacting protein A1U, Ataxin-1 interacting ubiquitin-like protein, UBQLN4, Ubiquilin-4
Фон	Ubiquilin-4 (UBQLN4) is a ~63 kDa protein. Regulator of protein degradation that mediates the proteasomal targeting of misfolded, mislocalized or accumulated proteins. Acts by binding polyubiquitin chains of target proteins via its UBA domain and by interacting with subunits of the proteasome via its ubiquitin-like domain. Key regulator of DNA repair that represses homologous recombination repair: in response to DNA damage, recruited to sites of DNA damage following phosphorylation by ATM and acts by binding and removing ubiquitinated MRE11 from damaged chromatin, leading to MRE11 degradation by the proteasome. MRE11 degradation prevents homologous recombination repair, redirecting double-strand break repair toward non-homologous end joining (NHEJ). Specifically recognizes and binds mislocalized transmembrane-containing proteins and targets them to proteasomal degradation. 1. Riley, BE. et al. (2004) The Journal of biological chemistry 279, 42290-301. PMID: 15280365 2. Suzuki, R. et al. (2016) EMBO reports 17, 842-57. PMID: 27113755 3. Hirayama, S. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, E4199-E4208. PMID: 29666234 4. Jachimowicz, RD. et al. (2019) Cell 176, 505-519.e22. PMID: 30612738 5. Lee, DY. et al. (2013) EMBO reports 14, 373-81. PMID: 23459205
Примечание	For research use only.

Изображения

SDS-PAGE

SDS-PAGE for Recombinant Human UBQLN4/Ubiquilin-4 Protein, N-GST & C-His

Ссылки

Даташит

Human UBQLN4/Ubiquilin-4 Recombinant Protein (N-GST & C-His).pdf

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