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| カタログ番号 | HF923012 |
|---|---|
| 説明 |
Recombinant Human USP19 Protein, N-His (HF923012) expressed in E. coli, spanning Lys286-Gln376. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | O94966 |
| タンパク質長 | Lys286-Gln376 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 13.10 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | Deubiquitinating enzyme 19, Ubiquitin thioesterase 19, KIAA0891, ZMYND9, Ubiquitin carboxyl-terminal hydrolase 19, Ubiquitin-specific-processing protease 19, Zinc finger MYND domain-containing protein 9, USP19 |
| 背景 | Ubiquitin carboxyl-terminal hydrolase 19 (USP19) is a ~145 kDa protein. Deubiquitinating enzyme that regulates the degradation of various proteins by removing ubiquitin moieties, thereby preventing their proteasomal degradation. Stabilizes RNF123, which promotes CDKN1B degradation and contributes to cell proliferation. Decreases the levels of ubiquitinated proteins during skeletal muscle formation and acts to repress myogenesis. Modulates transcription of major myofibrillar proteins. Also involved in turnover of endoplasmic-reticulum-associated degradation (ERAD) substrates. 1. Hassink, GC. et al. (2009) EMBO reports 10, 755-61. PMID: 19465887 2. Lee, JG. et al. (2014) The Journal of biological chemistry 289, 3510-7. PMID: 24356957 3. Mei, Y. et al. (2011) The Journal of biological chemistry 286, 35380-35387. PMID: 21849505 4. Altun, M. et al. (2012) The Journal of biological chemistry 287, 1962-9. PMID: 22128162 5. Chai, P. et al. (2021) The Journal of cell biology 220. PMID: 33978709 6. Yu, W. et al. (2024) Journal of Alzheimer's disease : JAD 100, 799-808. PMID: 38943386 7. Lei, CQ. et al. (2019) Journal of immunology (Baltimore, Md. : 1950) 203, 259-268. PMID: 31127032 8. Xue, W. et al. (2020) The Biochemical journal 477, 4295-4312. PMID: 33094816 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human USP19 Protein, N-His
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