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| カタログ番号 | HV501022 |
|---|---|
| 説明 |
Recombinant Human USP25 Protein, N-His (HV501022) expressed in E. coli, spanning Glu862-Arg1055. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q9UHP3 |
| タンパク質長 | Glu862-Arg1055 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 25.44 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | Ubiquitin-specific-processing protease 25, Ubiquitin thioesterase 25, USP21, USP on chromosome 21, Ubiquitin carboxyl-terminal hydrolase 25, Deubiquitinating enzyme 25, USP25 |
| 背景 | Ubiquitin carboxyl-terminal hydrolase 25 (USP25) is a ~122 kDa protein. Deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates and thus, functions in various biological processes including inflammation and immune response. Modulates the Wnt/beta-catenin pathway by deubiquitinating and stabilizing tankyrases TNKS1 and TNKS2. Regulates KEAP1-NRF2 axis in the defense against oxidative assaults by deubiquitinating KEAP1 and protecting it from degradation leading to degradation of the NRF2 transcription factor that is responsible for mounting an anti-oxidation gene expression program. Positively regulates RNA virus-induced innate signaling by interacting with and deubiquitinating ERLIN1 and ERLIN2. In turn, restricts virus production by regulating cholesterol biosynthetic flux. 1. Qian, G. et al. (2018) Biochemical and biophysical research communications 498, 537-543. PMID: 29518389 2. Teo, QW. et al. (2023) Developmental cell 58, 2495-2509.e6. PMID: 37683630 3. Xu, D. et al. (2017) Genes & development 31, 1024-1035. PMID: 28619731 4. Sauer, F. et al. (2019) Molecular cell 74, 421-435.e10. PMID: 30926243 5. Fan, CX. et al. (2024) Brain : a journal of neurology 147, 3442-3457. PMID: 38875478 6. Cai, C. et al. (2023) Nature communications 14, 3648. PMID: 37339955 7. Zhong, B. et al. (2012) Nature immunology 13, 1110-7. PMID: 23042150 8. Wen, J. et al. (2019) Molecular immunology 106, 53-62. PMID: 30579117 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human USP25 Protein, N-His
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