Anti-USP25 Polyclonal Antibody (HV501014)

Name: Anti-USP25 Polyclonal Antibody
Brand: abinScience
SKU: HV501014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HV501014

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human USP25 (Glu862-Arg1055).

Target

Ubiquitin-specific-processing protease 25, Ubiquitin thioesterase 25, USP21, USP on chromosome 21, Ubiquitin carboxyl-terminal hydrolase 25, Deubiquitinating enzyme 25, USP25

Purification

Purified by antigen affinity column.

Accession

Q9UHP3

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Ubiquitin carboxyl-terminal hydrolase 25 (USP25) is a ~122 kDa protein. Deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates and thus, functions in various biological processes including inflammation and immune response. Modulates the Wnt/beta-catenin pathway by deubiquitinating and stabilizing tankyrases TNKS1 and TNKS2. Regulates KEAP1-NRF2 axis in the defense against oxidative assaults by deubiquitinating KEAP1 and protecting it from degradation leading to degradation of the NRF2 transcription factor that is responsible for mounting an anti-oxidation gene expression program. Positively regulates RNA virus-induced innate signaling by interacting with and deubiquitinating ERLIN1 and ERLIN2. In turn, restricts virus production by regulating cholesterol biosynthetic flux.

1. Qian, G. et al. (2018) Biochemical and biophysical research communications 498, 537-543. PMID: 29518389

2. Teo, QW. et al. (2023) Developmental cell 58, 2495-2509.e6. PMID: 37683630

3. Xu, D. et al. (2017) Genes & development 31, 1024-1035. PMID: 28619731

4. Sauer, F. et al. (2019) Molecular cell 74, 421-435.e10. PMID: 30926243

5. Fan, CX. et al. (2024) Brain : a journal of neurology 147, 3442-3457. PMID: 38875478

6. Cai, C. et al. (2023) Nature communications 14, 3648. PMID: 37339955

7. Zhong, B. et al. (2012) Nature immunology 13, 1110-7. PMID: 23042150

8. Wen, J. et al. (2019) Molecular immunology 106, 53-62. PMID: 30579117

Note

For research use only.

Images

References

Datasheet

Anti-USP25 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-USP25 Polyclonal Antibody (HV501014)

Overview

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References

Datasheet

SDS

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