Human VPS41 Recombinant Protein (N-His) (HW488012)

Name: Human VPS41 Recombinant Protein (N-His)
Brand: abinScience
SKU: HW488012
Price: 211 USD
Availability: InStock

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1mg

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Número de catálogo	HW488012
Descripción	Recombinant Human VPS41 Protein, N-His (HW488012) expressed in E. coli, spanning Lys31-Asp361. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	P49754
Longitud de proteína	Lys31-Asp361
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Homo sapiens (Human)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	40.16 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	Vacuolar protein sorting-associated protein 41 homolog, VPS41, S53
Fondo	Vacuolar protein sorting-associated protein 41 homolog (VPS41) is a ~98 kDa protein. Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Acts as a component of the HOPS endosomal tethering complex. This complex is proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The HOPS complex is proposed to be recruited to Rab7 on the late endosomal membrane and to regulate late endocytic, phagocytic and autophagic traffic towards lysosomes. Involved in homotypic vesicle fusions between late endosomes and in heterotypic fusions between late endosomes and lysosomes implicated in degradation of endocytosed cargo. 1. Solinger, JA. et al. (2013) The FEBS journal 280, 2743-57. PMID: 23351085 2. van der Welle, REN. et al. (2021) EMBO molecular medicine 13, e13258. PMID: 33851776 3. Pols, MS. et al. (2013) Traffic (Copenhagen, Denmark) 14, 219-32. PMID: 23167963 4. Lin, X. et al. (2014) Scientific reports 4, 7282. PMID: 25445562 5. Khatter, D. et al. (2015) Journal of cell science 128, 1746-61. PMID: 25908847 6. Radisky, DC. et al. (1997) Proceedings of the National Academy of Sciences of the United States of America 94, 5662-6. PMID: 9159129 7. Wartosch, L. et al. (2015) Traffic (Copenhagen, Denmark) 16, 727-42. PMID: 25783203 8. Zhang, S. et al. (2023) Nature communications 14, 6360. PMID: 37821429 9. Garg, S. et al. (2011) Immunity 35, 182-93. PMID: 21802320
Nota	For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Recombinant Human VPS41 Protein, N-His

Referencias

Datasheet

Human VPS41 Recombinant Protein (N-His).pdf

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Formula

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