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| カタログ番号 | HT262012 |
|---|---|
| 説明 |
Recombinant Human WASL Protein, N-His (HT262012) expressed in E. coli, spanning Leu21-Arg148. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | O00401 |
| タンパク質長 | Leu21-Arg148 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 17.16 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | N-WASP, WASL, Neural Wiskott-Aldrich syndrome protein |
| 背景 | Actin nucleation-promoting factor WASL is a ~54 kDa protein. Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex. Involved in various processes, such as mitosis and cytokinesis, via its role in the regulation of actin polymerization. Together with CDC42, involved in the extension and maintenance of the formation of thin, actin-rich surface projections called filopodia. In addition to its role in the cytoplasm, also plays a role in the nucleus by regulating gene transcription, probably by promoting nuclear actin polymerization. Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression. 1. Wu, X. et al. (2006) Nature cell biology 8, 756-63. PMID: 16767080 2. Vingadassalom, D. et al. (2009) Proceedings of the National Academy of Sciences of the United States of America 106, 6754-9. PMID: 19366662 3. Zhang, J. et al. (2009) The Journal of biological chemistry 284, 21659-69. PMID: 19487689 4. Gaucher, JF. et al. (2012) The Journal of biological chemistry 287, 34646-59. PMID: 22847007 5. Aitio, O. et al. (2012) Structure (London, England : 1993) 20, 1692-703. PMID: 22921828 6. Miki, H. et al. (1998) Nature 391, 93-6. PMID: 9422512 7. Rajabian, T. et al. (2009) Nature cell biology 11, 1212-8. PMID: 19767742 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human WASL Protein, N-His
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